1.肝癌發生(hepato-carcinogenesis)與B型肝炎病毒感染之關係與分子機轉: B型肝炎病毒感染是導致肝癌很重要的原因。在癌症發生過程中，細胞常發生基因不穩定性(genomic instability)。因此維持基因的穩定與完整性對於預防癌症發生扮演重要的角色。我們發現B型肝炎病毒的表面抗原pre-S2突變種會造成基因不穩定性及造成DNA損害(DNA damage)。因此我們以B型肝炎病毒為study model，探討慢性B型肝炎帶原者在轉變成肝癌的過程中DNA damage與DNA repair機制的調控及其對HCC發生的影響。

2.DNA Repair 之分子病理: DNA Repair (DNA修復)是細胞內維持DNA結構完整及修復DNA損害最重要的一個機制。當生物體DNA受到環境途變原(environmental mutagens)例如x-ray，紫外線，以及有毒化學物質等刺激而引起DNA結構不正常時，就需靠DNA Repair 功能來使之恢復正常功能。DNA Repair 功能異常的病人由於DNA突變率較高，導致癌症的機率較正常人高出許多。我們主要的研究方向為探討DNA Repair的分子機轉及其調控，尤其是近年來才分離出的因子hHR23 A及hHR23B (human homolog of Rad23 A / B)，探討其在DNA Repair過程中所扮演的角色。除此之外，我們也利用微矩陣(Microarray)生物晶片技術來探討紫外線等環境途變原所引發的DNA Repair分子機轉。同時我們也進一步分析DNA Repair不正常病人的細胞株，以了解DNA Repair異常所引發的相關病理機制。

研究室成員

〔專案助理教授〕

姓名	研究主題
謝逸璇	Functional studies of DNA repair deficiency caused by hepatitis B virus pre-S2 mutant large surface antigen

〔助理〕

姓名	研究主題
沈芳晴	Hepatitis B virus large surface protein pre-S mutant on hepatocellular carcinoma
李彥陵	Human 8-oxoganine DNA glycosylase 1  isoform (-hOGG1) in mitochondrial DNA repair

〔行政助理〕

姓名	協助事項
吳慧真	實驗室器材整理、帳務整理

〔博士後研究員〕

姓名	研究主題
徐婕琳	The effects of hepatitis B virus large surface protein pre-S mutant on hepatocellular carcinoma

〔碩士班〕

姓名	論文題目
劉仁傑(醫技所二年級）	Genomic instability in the hepatitis B virus-induced hepatoma in transgenic mice
高慧齡(醫技所二年級）	Natural products to chemo-prevent HCC caused by the pre-S2 mutant LHBS
李芸萍(醫技所二年級）	Characterization of post-translational modifications of the human DNA repair factors OGG1 and NTH1
李宗翰(醫技所一年級）	Hepatitis B virus surface antigen HBsAg detection and differentiation: Large HBS and HBS
陳研玉(醫技所）

過去成員

〔大專生參與專案研究〕

姓名	研究主題
田偉廷(生命科學系)	Structural analysis of the pre-S region of the large HBS protein by NMR

〔已畢業博士〕

姓名	研究主題
謝逸璇(基醫所)-2007畢	Genomics instability induced by HBV Pre-S mutant surface proteins

〔已畢業碩士〕

姓名	論文題目
賴允婷 (分醫所)-2002畢	The protein interaction studies of the two human nucleotide excision repair factors hHR23A and hHR23B and their roles as protein degradation regulators
蔡瑞和 (醫工所)-2002畢	Application of cDNA microarray on studies of UVC-induced cell’s gene expressions
謝逸璇 (醫技所)-2003畢	Oxidative stress and genomic instabilities caused by the HBV pre-S mutant surface antigens
黃聿忻 (分醫所) -2003畢	Functional studies of the human hHR23A and hHR23B proteins in DNA repair and proteolysis
謝惠娟 (醫工所) -2004畢	Functional studies of DNA repair genes hHR23A and hHR23B
王雪閔 (醫技所)-2005畢	Genetic polymorphisms associated with breast cancer in south Taiwan
蔡漢霓 (醫技所)-2005畢	Studies of antioxidant activities in Agaricus blazei Murill extracts
王鈺婷 (分醫所)-2006畢	Association of mitochondrial function with chemo-resistance in lung cancer cells
許茹斐 (醫技所)-2006畢	Screening and functional studies of human proteins which interact with Streptococcal pyogenic exotoxin B (SPE B)
董秀蕙 (分醫所)-2007畢	Screening and functional studies of human proteins which interact with DNA-damage binding protein (DDB2)
黃玉君 (醫技所)- 2007畢	Functional studies of HBV surface antigen pre-S2 mutation with its association of JAB1 and KPNA2
李彥陵 (醫技所)-2009畢	Association of human 8-oxoguanine DNA glycosylase 1  isoform with NDUFB10 and its potential role in mitochondrial DNA repair
蘇郁虹(醫技所)-2010畢	Functional studies of the α- and β-hydroxyguanine glycosylases in mitochondrial DNA repair
阮玉鴛兒(醫技所)-2010畢	Microsatellite instability caused by pre-S2 mutant LHBS

照片

專利

• Huang, W., Su, I.-J., and Shen F.-C., 2009. “Oligonucleotides and use thereof for determining deletion in HBV pre-S region”. . US Patent Application.
• Chuang, W.-J., Fu, W.-M., Huang, T.-F., Huang, W., Tang, C.-H., and Chen, C.-Y., 2008. “Disintegrin variants and pharmaceutical uses thereof”. USA Patent Application Publication (20080188413A1).

技術轉移

• Chuang, W.-J., Fu, W.-M., Huang, T.-F., Huang, W., Tang, C.-H., and Chen, C.-Y., 2008. “Disintegrin variants and pharmaceutical uses thereof, USA Patent Application Publication (20080188413A1)”. Technology transfer to Anchen Pharmaceuticals (Taiwan, inc.)

Refereed Papers

• 2008~2011年
• 2005~2007年
• 2002~2004年
• 1997~2001年

2008~2011年

• Hsu, J.-F., Chuang, W.-J., Shiesh, S.-C., Liu, C.-C., Lin, Y.-S., Wang, C.-C., Fu, T.-F., Tsai, J.-H., Tsai, W.-L., Wu, J.-J., Lin, M.-T., and Huang, W. (corresponder), 2008. Streptococcal pyrogenic exotoxin B cleaved human S-adenosylhomocysteine hydrolase and induced hypermethioninemia. J. Infectious Diseases. 198, 367-374. (SCI)
• Su, I.-J., Wang, H.-C., Wu, H.-C., and Huang, W., 2008. Ground glass hepatocytes contain pre-S mutants and represent preneoplastic lesions in chronic hepatitis B virus infection. J. Gastroen. Hepat. 23, 1169-1174. (SCI)
• Tsai, P.-C., Guo, Y.-L. L., Tsai, J.-H., Wang, Y.-J., Ueng. T.-H., Liu, H.-S., and Huang, W. (corresponder). Reduced expression of von Hippel-Lindau gene exposed to polychlorinated biphenyls and dibenzofurans. Environ. Res. 108, 247-251. (SCI)
• Chang, W.-W., Su, I.-J., Chang, W.-T., Huang, W., and Lei, H.-Y., 2008. Suppression of p38 mitogen-activated protein kinase inhibits hepatitis B virus replication in human hepatoma cell: the antiviral role of nitric oxide. J Viral Hepat.15, 490-497. (SCI)
• Yang, J.-C., Teng, C.-F., Wu, H.-C., Tsai, H.-W., Chuang, H.-C., Tsai, T.-F., Hsu, Y.-H., Huang, W., Wu, L.-W., and Su, I.-J., 2009. Enhanced expression of vascular endothelial growth factor-A in ground glass hepatocytes and its implication in HBV hepatocarcinogenesis. Hapthology 49, 1962-1971.
• Wang, C.-C., Houng, H.-C., Chen, C.-L., Wang, P.-J., Kuo, C.-F., Lin, Y.-S., Wu, J.-J., Lin, M.-T., Liu, C.-C., Huang, W., and Chuang, W.-J., 2009. Solution structure and backbone dynamics of streptopain: Insight into diverse substrate specificity. J. Biol. Chem. 284, 10957-10967.
• Shen, F.-C., Su, I.-J., Wu, H.-C., Hsieh, Y.-H., Yao, W.-J., Young, K.-C., Chang, T.-C., Hsieh, H.-C., Tsai, H.-N., and Huang, W. (corresponder), 2009. A Pre-S Gene Chip to detect the pre-S deletions in the hepatitis B virus large surface antigen as a predictive marker for hepatoma risk in the chronic HBV carriers. J. Biomed. Sci., 16, 84-91.
• Chen, Y.-L., Su, I.-J., Cheng, H.-Y., Chang, K.-C., Lu, C.-C., Chow, N.-H., Ho, C.-L., and Huang, W. (corresponder), 2010. BIOMED-2 protocols to detect clonal immunoglobulin and T cell receptor gene rearrangements in B- and T-cell lymphomas in southern Taiwan. Leukemia & Lymphoma, 51, 650-655.
• Hung, J.-H., Yan, C.-W., Su, I.-J., Wang, H.-C., Lei, H.-Y., Lin, W.-C., Chang, W.-T., Huang, W., Lu, T.-J., Lai, M.-D., 2010. Hepatitis B virus surface antigen interacts with acid alpha-glucosidase and alters glycogen metabolism. Hepatol. Res., 40, 633-640.
• Huang, C.-H., Tsai, H.-N., Pen, R.-H., Hsieh, Y.-H., Chuang, W.-J., Hsu, G.-C., Huang, W. (co-corresponder), Chen, W.-J., 2010. Agaricus Blazei Murill Ameliorates Myocardial Ischemia-Reperfusion Injury. Acta Cardiologica Sinica. 26, 235-241.
• Hsieh, Y.-H., Hsu, J.-L., Su, I.-J., and Huang, W. (corresponder), 2011. Genomic instability caused by hepatitis B virus: into hepatoma inferno. Frontiers in Bioscience. 17, 2586-97.
• Chang, Y.-S., Tsai, C.-T., Huang-Fu, C.-A., Huang, W., Lei, H.-Y., Lin, C.-F., Su, I.-J., Chang, W.-T., Wu, P.-H., Chen, Y.-T., Hung, J.-H., Young, K.-C., and Lai, M.-D., 2011. ACSL3 and GSK-3β are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells. J Cell Biochem. 112, 881-893.
• Tsai, H.-W., Lin, Y.-J., Lin, P.-W., Wu, H.-C., Huang, W., and Su, I.-J., 2011. Extensive ground glass hepatocytes in the non-tumorous livers is a risk factor for the recurrence of hepatocellular carcinoma in chronic hepatitis B patients receiving hepatectomy. Cancer. In press.
• Teng, C.-F., Wu, H.-C., Tsai, H.-W., Shiah, H.-S., Huang, W., and Su, I.-J, 2011. Novel feedback inhibition of surface antigen synthesis by mTOR signal and its implication for hepatitis B virus tumorigenesis and therapy. Hepatology. In press.
• Li, M.-F., Hsiao, C.-H., Chen, Y.-L., Huang, W., and Lien, H.C. HHV-8/KSHV-associated lymphoma mimicking cutaneous T-cell anaplastic large cell lymphoma in a HIV-positive patient. In revision.
• Hsieh, Y.-H., Su, I.-J., Tsai, T.-F., Kuo, H.-L., Shen, F.-C., Kao, L.-Y., Huang, Y.-J., Hsieh, W.-C., Tsai, H.-N., Huang, W. (corresponder). Histone deacetylase inhibitor suberoylanilide hydroxamic acid causes remission of hepatitis B virus pre-S2 mutant large surface antigen-induced oncogenic effects. Submitted.
• Lee, Y.-L., Huang, W., and Huang, C.-Y. Yeast 2-hybrid analyses of 2 forms of PriB in Klebsiella pneumoniae: Role of the conserved N- and C-terminal regions in the PriB family. Submitted.
• Wang, H.-C., Lo, Y.-C., Huang, W., Lai, M.-D., and Su, I.-J. Hepatitis B virus pre-S2 mutant promotes hepatocarcinogenesis via aberrant cyclin A induction and centrosome overduplication. Submitted.
• Su, W.-P., Lo, Y.-C., Wang, Y.-T., Huang, W., Lin, C.-C., Chen, H. H. W., Lai, W.-W., and Su, W.-C. Expression of uncoupling protein 2 affects the paclitaxel-induced reactive oxygen species generation and Stat3 activation in lung cancer cells. Submitted.
• Lo, Y.-C., Wu, H.-C., Wang, H.-C., Huang, W., and Su, I.-J. Differential transmembrane topology of hepatitis B virus pre-S deletion mutants in endoplasmic reticulum and its biologic significance. Submitted.

2005~2007年

• Wang, H.-C., Chang, W.-T., Chang., W.-W., Huang, W., Lei, H.-Y., Lai, M.-D., , Fausto, N., and Su, I.-J., 2005. Upregulation of cyclin A and nodular proliferation of hepatocytes induced by a pre-S2 deletion mutant in chronic HBV infection. Hepatology, 41, 761-770 (SCI)
• Hsieh, H.-C., Hsieh, Y.-H., Huang, Y.-H., Shen, F.-C., Tsai, H.-N., Tsai, J.-H., Lai, Y.-T., Wang, T., Chuang, W.-J., and Huang, W. (corresponder), 2005. HHR23A, a human homolog of the S. cerevisiae Rad23, regulates xeroderma pigmentosum C protein and is required for nucleotide excision repair. Biochem. Biophy. Res. Comm., 335, 181-187 (SCI)
• Chang, W.-W., Su, I.-J., Lai, M.-D., Chang, W.-T., Huang, W. and Lei, H.-Y., 2005. Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus infection. World J. Gastroenterology, 11, 6631-6637. (SCI)
• Cheng, T.-L., Chang, W.-W., Su, I.-J., Lai, M.-D., Huang, W., Lei, H.-Y. and Chang, W.-T., 2005. Therapeutic inhibition of hepatitis B virus surface antigen expression by RNA interference. Biochem. Biophy. Res. Comm., 336, 820-830 (SCI)
• Chen, C.-Y., Cheng, C.-H., Chen, Y.-C., Lee, J.-C., Chou, S.-H., Huang, W. and Chuang, W.-J., 2006. Preparation of amino-acid-type selective isotope labeling of protein expressed in Pichia pastoris. Proteins, 62, 279-287 (SCI)
• Tsai, P.-C., Huang, W., Lee, Y.-C., Chan, S.-H., and Guo, Y.-L., 2006. Genetic polymorphisms in CYP1A1 and GSTM1 predispose humans to PCBs/PCDFs-induced skin lesions. Chemosphere. 63, 1410-1418 (SCI)
• Chang, T.-W., Wang, S.-M., Guo, Y.-L., Tsai, P.-C., and Huang, W. (corresponder), 2006. Glutathione S-transferase polymorphisms associated with risk for breast cancers in southern Taiwan. The Breast, 15, 754-761. (SCI)
• Wang, H.-C., Huang, W., Lai, M.-D., and Su, I.-J., 2006. Hepatitis B virus pre-S mutants, endoplasmic reticulum stress and hepatocarcinogenesis. Cancer Sci. 97, 683-688 (SCI)
• Tsai, P.-C., Ko, Y.-C., Huang, W., Liu, H.-S., and Guo, Y.-L., 2007. Increased liver and lupus mortalities in 24-year follow-up of the Taiwanese people highly exposed to polychlorinated biphenyls and dibenzofurans. Sci. Total Environ. 374, 216-222. (SCI)
• Hsieh, Y.-H., Chang, W.-W., Su, I.-J., Lei, H.-Y., Lai, M.-D., and Huang, W. (corresponder), 2007. Differential ER stress signaling pathways mediated by inducible nitric oxide synthase. Biochem. Biophy. Res. Comm., 359, 643-648 (SCI)
• Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Tsai, J.-H., Chang, W.-W., Lai, M.-D., Lei, H.-Y., and Huang, W. (corresponder), 2007. Hepatitis B virus pre-S2 mutant surface antigen induces degradation of cyclin-dependent kinase inhibitor p27Kip1 through c-Jun activation domain-binding protein 1. Mol. Cancer Res., 5, 1063-1072. (SCI)

2002~2004年

• Guo, Y.-L. L., Chang, S.-C., Tsai, R.-H., Huang, J.-C., Li, C., Young, K.-C., Wu, L.-W., Lai, M.-D., Liu, H.-S. and Huang, W. (corresponder), 2002. Two UVC-induced stress response pathways in HeLa cells identified by cDNA microarray. Environ. Mol. Mutag., 40, 122-128. (SCI)
• Chuang, S.-S., Lin, C.-N., Shen, F.-C., Liao, P.-J., Liao, Y.-L., Chang, J. H., Tsai, Y.-C., Cho, C. Y., Huang, W. (corresponder), 2003. Detecting clonal rearrangements in non-Hodgkin’s lymphomas in Taiwan by polymerase chain reaction. Leukemia and Lymphoma, 44 (1), 117-121 (SCI)
• Chang, H.-C., Tsai, J.-H., Guo, Y.-L. L., Tsai, P.-C., Li, C. and Huang, W. (corresponder), 2003. Differential UVC-induced gadd45 gene expression in xeroderma pigmenstosum cells. Biochem. Biophy. Res. Comm., 305, 1109-1115 (SCI)
• Hsu, P. C., Huang, W., Yao, W.-J., Wu, M.-H., Guo, Y.-L. and Lambert, G. H, 2003. Sperm changes in humans exposed to polychlorinated biphenyls and dibenzofurans. JAMA, 289, 2943-2944 (SCI)
• Chang, W.-W., Su, I.-J., Lai, M.-D., Chang, W.-T., Huang, W., and Lei, H.-Y., 2003. The role of inducible nitric oxide synthase in a murine acute HBV infection model induced by hydrodynamics-based in vivo transfection of HBV-DNA. J. Hepatology, 39, 834-842. (SCI)
• Chuang, C.-J., Ma, M.-C., and Huang, W. 2003. On the evaluation of different statistical procedures for microarray data. J. Chinese Stat. Assoc., 41, 421-441
• Tsai, W.-C., Tsai, S.-T., Ko, J.-Y., Jin, Y.-T., Li, C., Huang, W., Young, K.-C., Lai, M.-D., Liu, H. S.,and Wu, L.-W., 2004. The mRNA expression profile in betel-quid chewing oral cancer patients. Oral Oncology, 40, 418-426. (SCI)
• Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Chang., W.-W., Lei, H.-Y., Lai, M.-D., Chang, W.-T., and Huang, W. (corresponder), 2004. The pre-S mutant surface antigens in chronic hepatitis B virus infection induce oxidative stress and DNA damage. Carcinogenesis, 25,2023-2032 (SCI)
• Hung, J.-H., Su, I.-J., Lei, H.-Y., Wang, H.-C., Lin, W. C., Chang, W.-T., Huang, W., Chang, W. C., Chang, Y. S., Chen, C.-C., and Lai, M. D. 2004. Endoplasmic reticulum stress stimulates the expression of cyclooxygenase-2 through activation of NF- B and pp38 mitogen-activated protein kinase. J. Biol. Chem., 279, 46384-46392 (SCI)

1997~2001年

• Huang, W. and P. D. Smith, 1997. The mus206 gene of Drosophila melanogaster is required for the excision repair of alkylation-induced DNA lesions. Mutation Research: 384 (2), 81-88 (SCI)
• Huang, W., W. J. Feaver, A. Tomkinson, and E. C. Friedberg, 1998. The N-degron protein degradation strategy for investigating the function of essential genes: requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts. Mutation Research, 408 (3), 183-194 (SCI)
• Rodriguez, K., J. Talamantrez, W. Huang, S. H. Reed, Z. Wang, L. Chen, W. J. Feaver, E. C. Friedberg and A. E. Tomkinson, 1998. Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein. J. Biol. Chem., 273, 34180-34189 (SCI)
• Russell, S. J., S. H. Reed, W. Huang, E. C. Friedberg and S. A. Johnston, 1999. The 19S regulatory complex of the proteasome functions independently of proteolysis in nucleotide excision repair. Molecular Cell, 3, 687-695 (SCI)
• Feaver, W.J., W. Huang, E. C. Friedberg, 1999. The TFB4 subunit of yeast TFIIH is required for both nucleotide excision repair and RNA polymerase II transcription. J. Biol. Chem., 274 (41), 29564-29571 (SCI)
• Feaver, W. J., W. Huang, O. Gileadi, L. Myers, C. M. Gustafsson, R. D. Kornberg and E. C. Friedberg, 2000. Subunit interactions in yeast transcription/repair factor TFIIH: Requirement for Tfb3 subunit in nucleotide excision repair. J. Biol. Chem., 275 (8), 5941-5946. (SCI)
• Gillette, T. G., W. Huang, S. J. Russell, S. H. Reed, S. A. Johnston and E. C. Friedberg, 2001. The 19S complex of the proteasome regulates nucleotide excision repair in yeast. Genes and Development, 15, 1528-1539 (SCI)
• Chen, C.-C., Shieh, B., Jin, Y.-T., Liau, Y.-E., Huang, C.-H., Liou, J.-T., Wu, L.-W., Huang, W., Young, K.-C., Lai, M.-D., Liu, H.-S. and Li, C., 2001. Microarray profiling gene expression patterns in bladder tumor cells treated with genistein. J. Biomed. Sci., 8, 214-222 (SCI)
• Chuang, S.-S., Huang, W., Lin, C.-N., Chio, C.-C., Tsai, T.-C., Li, C.-Y., Shen, C.-H., 2001 Primary cerebral anaplastic large cell lymphoma containing abundant reactive histiocytes and eosinophils: a case report and literature review. Pathol. Res. Pract., 197, 647-652 (SCI)

Conference papers

• 2008~2011年
• 2006~2007年
• 2001~2005年
• 1993~1999年

2008~2011年

• Huang, W. (corresponder), Chen, Y.-L., Cheng, H.-Y., Lu, C.-C., Ho, C.-L., 2008. Molecular diagnosis of clonal immunoglobulins and T cell receptor gene rearrangements in lymphomas by BIOMED-2 protocols. Asia-Pacific Medical Laboratory Science Forum, Taichung, Taiwan.
• Huang, W. (corresponder), Hsieh, Y.-H., Hsieh, H.-C., and Su, I.-J., 2008. Inhibition of DNA repair by the hepatitis B virus large surface antigen pre-S2 mutant. American Association for Cancer Research, San Diego, USA.
• Su, W.-P., Huang, W., Lo, Y.-C., Wang, Y.-T., and Su, W.-C., 2008. Paclitaxel activates Stat3 via mitochondria-mediated reactive oxygen species (ROS) production in lung cancer cells. American Association for Cancer Research, San Diego, USA.
• Tsai, H.-N., Hsieh, H.-C., Hsieh, Y.-H., Su, I.-J., and Huang, W. (corresponder), 2008. Inhibition of DNA repair by the hepatitis B virus large surface antigen pre-S2 mutant. International Symposia in Signal Transduction and Gene Regulation, Tainan, Taiwan.
• Shen, F.-C., Su, I.-J., Wu, H.-C., and Huang, W. (corresponder), 2008. The Pre-S Gene Chip to detect the pre-S deletions in the hepatitis B virus large surface antigen - a predictive method for HCC risk in the chronic HBV carriers. International Symposia in Signal Transduction and Gene Regulation, Tainan, Taiwan.
• Huang, W. (corresponder), Chen, Y.-L., Cheng, H.-Y., Su, I.-J., Lu, C.-C., Chow, N.-H., and Ho, C.-L., 2008. Molecular Diagnosis of Clonal Immunoglobulin and T Cell Receptor Gene Rearrangements in B- and T-cell Lymphomas in Taiwan. Annual Meeting in Medical Technology, Taipei, Taiwan.
• Shen, F.-C., Su, I.-J., Wu, H.-C., and Huang, W. (corresponder), 2009. The Pre-S Gene Chip to detect the pre-S deletions in the hepatitis B virus large surface antigen - a predictive method for HCC risk in the chronic HBV carriers. Annual meeting for Molecular and Cellular Biology, Kenting, Taiwan.
• Lee, Y.-L, and Huang, W. (corresponder), 2009. Human 8-oxoganine glycosylase 1  isoform (OGG1) in mitochondrial DNA repair. American Association for Cancer Research, Denver, USA.
• Huang, W. (corresponder), Hsu, J.-L., Hsieh, Y.-H., Hsieh, H.-C., Tsai, H.-N., and Su, I.-J., 2009. Inhibition of DNA repair by the hepatitis B virus large surface antigen pre-S2 mutant. HBV Molecular Biology Annual Meeting, Tours, France.
• Chen, Y.-L., Huang, W., Cheng, H.-Y., Su, I.-J., Lu, C.-C., Chow, N.-H., Ho, C.-L., 2009. Molecular Diagnosis of Clonal Immunoglobulin and T Cell Receptor Gene Rearrangements in B- and T-cell Lymphomas in south Taiwan. Asia-Pacific Medical Laboratory Science Forum, Kaoshiung, Taiwan.
• Tsai, H.-N., Hsieh, Y.-H., Lin, Y.-T., Su, I-J., and Huang, W. (corresponder), 2010. Histone deacetylase inhibitor SAHA as a chemopreventive drug for hepatoma induced by hepatitis B virus pre-S2 mutant large surface antigen. Annual meeting for Molecular and Cellular Biology, Kenting, Taiwan.
• Su, Y.-H., M., Lee, Y.-L., Tian, W.-T., and Huang, W. (corresponder), 2010. Functional studies of the α- and β-human 8-oxoguanine DNA glycosylase 1 in mitochondrial DNA repair. Annual meeting for Molecular and Cellular Biology, Kenting, Taiwan.
• Cheng, H.-Y., Chen, Y.-L., Luo, C.-Y., Ho, C.-L., and Huang, W. (corresponder), 2010. Detection of genetic polymorphisms associated with warfarin susceptibility by high-resolution melting curve analysis. Asia-Pacific Medical Laboratory Science Forum, Taipei, Taiwan.
• Huang, W. (corresponder), Hsieh, Y.-H., and Su, J.-J., 2010. The hepatitis B virus pre-S2 mutant large surface antigen in hepatocellular carcinogenesis: molecular mechanisms and diagnosis. HBV Molecular Biology Annual Meeting, Taipei, Taiwan.
• Nguyen, N. U. H., Shen, F.-C., Hsu, J.-L., Tsai, H.-W., Su, I.-J., and Huang, W. (corresponder), 2010. Characteristics of genomic instability caused by hepatitis B virus Pre-S2 mutant large surface antigen. HBV Molecular Biology Annual Meeting, Taipei, Taiwan.
• Kao, H.-L., Hsieh, Y.-H., Su, I. J., and Huang, W. (corresponder), 2010. Histone deacetylase inhibitor sulforaphane to chemo-prevent hepatoma in chronic HBV carriers. HBV Molecular Biology Annual Meeting, Taipei, Taiwan.
• Lin, Y.-T., Yang, J.-C., Hsieh, W.-C., Su, I.-J., and Huang, W. (corresponder), 2010. Analysis of global genetic and transcriptional changes caused by hepatitis B virus pre-S2 mutant large surface antigen. HBV Molecular Biology Annual Meeting, Taipei, Taiwan.
• Hsieh, Y.-H., Kao, H.-L., Shen, F.-C., Tsai, H.-N., and Huang, W. (corresponder), 2010. Histone deacetylase inhibitor SAHA as a chemopreventive drug for hepatoma induced by hepatitis B virus pre-S2 mutant large surface antigen. HBV Molecular Biology Annual Meeting, Taipei, Taiwan.
• Liou, R.-J., Su, I.-J., Hsieh, W.-C., and Huang, W. (corresponder), 2011. Genomic stability-related gene expression changes in the hepatitis B virus-induced hepatoma in transgenic mice. International conferences on Signal Transduction and Gene Regualtion. Tainan, Taiwan.

2006~2007年

• Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Tsai, J.-H., Chang, W.-W., Lai, M.-D., Lei, H.-Y. and Huang, W. (corresponder), 2006. Hepatitis B virus pre-S2 mutant surface antigen induces retinoblastoma hyperphosphorylation through interaction with JAB1 protein in endoplasmic reticulum: a novel mechanism for HBV-related hepatocarcinogenesis. 5th NHRI conference on viral hepatitis and hepatocellular carcinoma. Taipei.
• Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Tsai, J.-H., Chang, W.-W., Lai, M.-D., Lei, H.-Y. and Huang, W. (corresponder), 2006. Functional regulation of cyclin-dependent kinase p27Kip1 by hepatitis B virus large surface antigen pre-S2 mutant. 5th NHRI conference on viral hepatitis and hepatocellular carcinoma. Taipei.
• Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Tsai, J.-H., Chang, W.-W., Lai, M.-D., Lei, H.-Y. and Huang, W. (corresponder), 2006. Hepatitis B virus pre-S2 mutant surface antigen induces retinoblastoma hyperphosphorylation through interaction with JAB1 protein in endoplasmic reticulum. The 27th annual meeting for American Association for Cancer Research, MD, USA.
• Huang, W. (corresponder), Hsieh, Y.-H., Chang, W.-W., Su, I.-J., Wang, H.-C., and Lei, H.-Y., 2006. Differential endoplasmic reticulum stress signaling pathway mediated by inducible nitric oxide synthase. The 27th annual meeting for American Association for Cancer Research, MD, USA.
• Hsieh, Y.-H., Su, I.-J., and Huang, W. (corresponder), 2007. Repression of HBV surface antigen pre-S2 mutant-induced genomc instabilities by histone deacetylase I inhibitors. The 28th annual meeting for American Association for Cancer Research, MD, USA.
• Huang, Y.-J., and Huang W. (corresponder), 2007. Identification of functional domains for interactions between JAB1 and the hepatitis B virus surface antigen pre-S2 mutant. Annual Meeting in Biophysics, Taipei.
• Huang, W. (corresponder), Hsieh, Y.-H., and Su, I.-J., 2007. Genomic Instabilities Caused by Hepatitis B Virus Large Surface Antigen Pre-S2 Mutant. 2007 International meeting on “The Molecular Biology of Hepatitis B Virus”, Rome, Italy.
• Tung, H.-H., and Huang, W. (corresponder), 2007. Functional studies of the human damaged-DNA binding protein 2 (DDB2) and its associated protein B cell early response gene (BERG36). Biomedical Annual Meeting, Taipei.

2001~2005年

• Huang, W. (corresponder), Hsieh, Y.-H., Su, I.-J., Wang, H.-C., Chang., W.-W., Lei, H.-Y., Lai, M.-D., Chang, W.-T., and, 2005. The pre-S mutant surface antigens in chronic hepatitis B virus infection induce oxidative stress and DNA damage. The 26th annual meeting for American Association for Cancer Research, CA, USA.
• Huang, W. (corresponder), Tsai, H.-N., and CHuang, W.-J. 2004. Anti-tumorigenic activity by Agaricus blazei Murill. Annual meeting in Medical Technology, Taipei.
• Tsai, P.-C., Huang, W., Lee, Y.-C. and Guo, Y.-L., 2004. Expression of von Hippel Lindau gene as exposure and outcome marker for PCB/PCDF exposed population. Epidemiology. 15, S74.
• Huang, W.; Tsai, P.-C. and Guo, Y.-L., 2004. Multiple gene polymorphisms as human susceptibility factors to multiple outcomes of persistent organic pollutants (POPS). Epidemiology, 15, S73-S74.
• Huang, W. (corresponder), Chang, T.-W., Wang, S.-M., and Guo, Y.-L., 2004. Single nucleotide polymorphisms associated with breast cancer in Southern Taiwan. Annual meeting in Medical Technology, Taipei.
• Huang, W. (corresponder), Hsieh, Y.-H., Wang, H.-C., Lei, H. Y., Lai; M.-D., Chang, W.-C., and Su, I.-J., 2004. The Pre-S mutant HBS antigens in chronic HBV infection induce oxidative stress and genomic instability. 4th NHRI conference on viral hepatitis and hepatocellular carcinoma. Taipei.
• Wang, S.M., Chang, T. W., Huang, W. (corresponder), 2004 The association between BRCA2 polymorphisms and breast cancer. The 19th Biomedical Conference, Taipei.
• Hsieh, Y. H., Su, I.-J., Lei, H.-Y., Lai, M.-D., Chang, W.-T. and Huang, W. (corresponder), 2003 Genomic stress and mutations caused by pre-S2 mutant hepatitis B virus surface antigen. The 18th Biomedical Conference, Taipei.
• Hsu P.C., Huang, W., Yao, W.J., Wu, M.H., Guo, Y.L., and Lambert, G.H. (2003) Comparison of the changes of sperm function between humans prenatally and postnatally exposed to Polychlorinated Biphenyls and Dibenzofurans. The 23th International Symposium on Halogenated Environmental Organic Pollutants and Persistent Organic Pollutants, Boston, USA.
• Lai, Y.-T., Wu, L.-C., Wang, T. and Huang, W. (corresponder), 2002. Protein-protein interactions for the human nucleotide excision repair factors hHR23A and hHR23B. EMS Annual Meeting.
• Guo, Y.L., Hsu, P.C., Huang, W., and Yao, W.J. (2002) “Sperm changes in men exposed to polychlorinated biphenyls and dibenzofurans”, The 17th Asia Conference on Occupational Health, November 1-3, Taipei, Taiwan
• Tsai, R.-H., Guo, Y.-L, L., Chang, H.-C., Li, C. and Huang, W. (corresponder), 2001. Dioxin-induced gene expression by the cDNA microarray analysis. Ninth symposium on recent advances in cellular and molecular biology, Taiwan.
• Lai, Y.-T., Wu, L.-C., Hsu, Y.-Y. and Huang, W. (corresponder), 2001. A simple approach to hHR23A and B, two human homologs of S. cerevisiae DNA repair gene Rad23. Ninth symposium on recent advances in cellular and molecular biology, Taiwan.
• Huang, J. C., Guo, Y. L. L., Li, C. and Huang, W. (corresponder), 2001. Ultraviolet light-induced gene expression by cDNA microarray analysis. EMS Annual Meeting.

1993~1999年

• Gillette, T. G., Huang, W., Russell, S. J., Reed, S. H. and Friedberg, E. C., 1999. Rescue of nucleotide excision repair (NER) activity in rad23 mutant strains by mutations in the 19S regulatory complex of the yeast proteasome. ASM conference on "DNA Repair and Mutagenesis". P. 73
• Huang, W., Russell, S. J., Reed, S. H, Johnston, S. A. and Friedberg, E. C., 1999. Identification of Sug1 and Sug2 subunits of the 19S regulatory complex of proteasome as suppressors of the NER factor Rad23 in S. cerevisiae. ASM conference on "DNA Repair and Mutagenesis". P. 73
• Huang, W., Wei, S., Tomkinson, A. E., Cullmann, G., Stillman, B. and Friedberg, E. C., 1998. The roles of RPA and PCNA proteins in nucleotide excision repair in the yeast Saccharomyces cerevisiae. Conference on “DNA Repair: Bacteria to Humans”. p. 119
• Huang, W. Y. and Smith, P. D., 1995. Large scale isolation of DNA repair genes from Drosophila melanogaster by PlacW single insertional mutagenesis. Conference on Repair and Processing of DNA Damage, 1995 Keystone Symposia on Molecular and Cellular Biology. I. Cell. Biochem., supp. 21A, p.298
• Huang, W. Y. and Smith, P. D., 1995. Characterization of a Drosophila DNA repair gene by PlacW single insertional mutagenesis. 26th Annual Scientific meeting of Environmental Mutagen Society. Environ. Mol. Mutag., 25, supp. 25, p. 24
• Huang, W. Y., Uptain, S. M., Shehab, T., Dusenbery, R. L. and Smith, P. D., 1993. The mus206 gene of Drosophila melanogaster is defective in excision repair of alkylation damage. 24th Annual Scientific Meeting of Environmental Mutagen Society. Environ. Mol. Mutag., 21, supp. 22, p. 31

Books

• Friedberg, E. C., W. J., Feaver, W. Huang, M. S. Reagan, S. H. Reed, Z. You, S. Wei, K. Rodriguez, J. Talamantez and A. E. Tomkinson, 1999. The current status of nucleotide excision repair in the yeast Saccharomyces cerevisiae. In Advances in DNA Damage and Repair : Oxygen Radical Effects, Cellular Protection, and Biological Consequences, edited by M. Dizdaroglu and A. E. Karakaya, Plenum Publishing Corp., New York
• Friedberg, E. C., W. J. Feaver, W. Huang, M. S. Reagan, W. A. Ramos, K. Rodriguez, S. H. Reed, A. E. Tomkinson, S. Wei and Z. You, 1998. Nucleotide excision repair in yeast: recent progress and implications. In DNA Repair, edited by F. Eckstein and D. M. J. Lilley, Springer Verlag, New York

作品發表(含展演)

Awards

• Research Grant Award. Hsieh, H.-C. and Huang, W. 2007. Inhibition of homologous recombination by pre-S2 HBsAg mutants and its association with hepatocellular carcinogeneis. Chinese Hepatitis Prevention Foundation. Taiwan.
• Research Grant Award. Hsieh, Y.-H. and Huang, W. 2004. Study of pre-S HBsAg mutants in genomic instability. Chinese Hepatitis Prevention Foundation. Taiwan.
• Poster award. Hsieh, Y. H., Su, I.-J., Lei, H.-Y., Lai, M.-D., Chang, W.-T. and Huang, W. (corresponder), 2003 Genomic stress and mutations caused by pre-S2 mutant hepatitis B virus surface antigen. The 18th Biomedical Conference, Taipei.

Journal reviewers

• Future Virology, 2008.
• 2Carcinogenesis, 2007.
• Oncogene, 2006
• FEBS Letters, 2006.
• Journal of Viral Hepatitis, 2005.
• Journal of Biomedical Sciences, 2004.

Other publications

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