陳淑慧(Shu-Hui Chen)                     分析化學    Analytical Chemistry
   生化分析研究室  Separation Science Laboratory

 學經歷

      B.S. in Chemistry, National Tsing Hua University.(1983)

      M.S. in Chemistry, National Taiwan University.(1986)

      Ph.D., Michigan State University, USA.(1993)

      Postdoctoral Associate, Fox Chase Cancer Center, Philadelphia, USA.(1994-96)

      Associate Professor, National Cheng Kung University.(1996)

   

 研究興

       本研究室目前著重於生化高分子化合物的分離。研究方

     向包括 :

  1.藉由毛細管柱上的直接偵測,對蛋白質在液相層析分離

    過程中結構的改變作探討。

  2.應用毛細管電泳來作DNA 類藥物及致病基因在生物樣品

    中的定量分析。

  3.以電腦模擬並最適化生化高分子化合物在分析及製備級管

    柱的分離條件。 以上的研究目標在建立高靈敏度,

    效,及彈性的分離策略以應在生化分離上的廣大需求。

 Research Interests 

      The research interest in this laboratory is focused on the separation of biomolecules. Current directions include:
 1. Investigation of the change of protein conformation during chromatographic separation via in-situ detection 

     along optically transparent capillary column. 

 2. Application of capillary electrophoresis for the quantitative analysis of DNA-based drugs and

     disease-causing genes in biological fluids.

 3. Computer simulation and optimization of the separation and purification of biomolecules in analytical as well as

     in preparative columns.  The goal of the research directions is to establish sensitive, efficient and flexible 

     analytical strategies towards the enormous demands in bio-separation. normal and sugar or uv induced cataract rens.

 Selected Publications

 

1. "Photodissociation of Br2 and the Electronic Raman spectra of Br Under Argon Ion Laser Light". J. Raman Spectrosc., 1986, 17, 453.

2. "Selective Fluorescence Quenching of Polynuclear Aromatic Hydrocarbons in Microcolumn Liquid Chromatography". Anal. Chim. Acta, 1991, 246, 65.

3. "Direct Examination of Separation Processes in Liquid Chromatography: Effect of Temperature and Pressure on Solute Retention". J. Microcol. Separ., 1993, 5, 3.

4. "Temperature Effect on Pyrene as A Polarity Probe for Supercritical Fluid and Liquid Solutions". Appl. Spectrosc., 1994, 48, 596.

5. “Pharmacokinetics and Tissue Distribution of a DNA-methyltransferase Antisense (MT-AS) Oligonucleotide and Its Catabolites in Tumor Bearing Nude Mice”. Journal of Pharmacology and Experimental Therapeutics, 1997, 663, 282.

6. "Determination of Antisense Phosphorothioate Oligonucleotides and Catabolites in Biological Fluids and Tissue Extracts Using Anion Exchange Chromatography and Capillary Electrophoresis". J. Chromatogr. B Biomed. Appl, 1997, 692, 43.  

7. "Experimental and Theoretical Studies of Pressure in Liquid Chromatography ". Anal. Chem., 1997, 69, 930.  

8. "The Molar Volume and Molar Enthalpy of Methylene and Benzene Homologues in Reversed-Phase Liquid Chromatography". J. Chromatogr. A, 1997, 762, 35.

9. “Use of CE Methods to Characterize the Pharmacokinetics of Antisense Drugs”. Electrophoresis, 1998, 19, 2861-2869.

10. “Plastic Microchip Electrophoresis for Analysis of PCR Products of Serum Hepatitis C Virus” Clin. Chem., 1999, 45, 1938-1943.

11. “Pharmacokinetic application of capillary electrophoresis”. Electrophoresis, 1999, 20, 3259-3268.

12. “Determination of fatty acids in vegetable oil by reversed-phase liquid chromatography with fluorescence detection”. J. Chromatogr. A, 1999, 849, 357-369.  

13. “Polymer Solution-Filled Column for the Analysis of Antisense Phosphorothioates by Capillary Electrophoresis”. Electrophoresis, 1999, 20, 547-554. 

 14. “The use of microchip electrophoresis for the analysis of PCR products” LC-GC Magazine (Europe), 2000, 13, 766-773. 

15. “Pressure-induced retention of the lysozyme on reversed-phase liquid chromatography” J. Chromatogr. A, 2000, 891/2, 207-215. 

16. “Quantitative Detection of N7-(2-hydroxyethyl)guanine Adducts in DNA Using High Performance Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry” J. Mass. Spec., 2001, 36, 336-343.

17. “Microfabricated Plastic Chips by Hot Embossing Methods and Their Applications for DNA Separation and Detection “Sensors & Actuators B: Chemical, 2001, 75 (1-2), 142-148.

18. “Plastic Microchip Electrophoresis for Genetic Screening – The Analysis of PCR Products of Fragile X(CGG)n Alleles” Electrophoresis, 2001, 22, 1188-1193.

19. “Microfabricated Electrophoresis Chips on Quartz Substrates and Their Applications on DNA Analysis” J. Chin. Chem. Soc., 2001, 48, 1123-1128.

20. “A disposable poly(methylmethacrylate)-based microfluidic module for protein identification by nano-electrospray ionization tandem mass spectrometry” Electrophoresis, 2001, 22, 3972-3977.

21. “Recent Advances in Pharmacokinetic Applications of Capillary Electrophoresis” Electrophoresis, 2001, 22, 4244-4248.

22. “Flow-through sampling for electrophoresis-based microfluidic chips using hydrodynamic pumping” J. Chromatogr. A, 2001, 937/1-2, 115-125.

23. “Microchip electrophoresis and the Analysis of Polymerase Chain Reaction Products” LC-GC Magazine (North America), 2002, 20 (2), 164-173.

24. “A reverse-transcription Competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA” J. Virological Methods, 2002, 103, 27-39.

25. “Microchip and Capillary Electrophoresis for Quantitative Analysis of Hepatitis C Virus based on RT- competitive PCR” Talanta, 2002, 56, 323-330.

26. “Analysis of fatty acids by column liquid chromatography” Anal. Chim. Acta, 2002, 465, 145-155.

27. “Automation for Continuous Analysis on Microchip Electrophoresis using Flow-Through Sampling” Electrophoresis, 2002, 23, 3550-3557.

28. “Flow-through sampling for electrophoresis-based microchips and their applications for protein analysis” Anal. Chem., 2002, 74, 5146-5153. 

29. “Micro Capillary Electrophoresis Chips Integrated with Buried SU-8/SOG Optical Waveguides for Bio-analytical Applications” Sensors & Actuators A:, 2003, 107, 125-131.

30. “Poly (dimethylsiloxane) (PDMS)-Based Microfluidic Device with ESI-MS Interface for Protein Identification” Electrophoresis, 2003, 24, 3648-3654.

31. “Recent Progress in Pharmacokinetic Applications of Capillary Electrophoresis” Electrophoresis, 2003, 24, 4106-4115.

32. “Dimethyl Stable-Isotope Labeling for Quantitative Proteomics” Anal. Chem., 2003, 75, 6843-6852.

33. “Prolongation of Heart Allograft Survival of Rats Treated by a Th2 Inhibitor” Transplant Immunology, 2003, 11, 385-388.

34. “Thermodynamic Studies of Pressure-induced Retention of Peptides in Reversed-Phase HPLC” J. Chromatogr. A., 2004, 1023, 41-47.

35. “Integrated Optical Fiber Capillary Electrophoresis Microchips with Novel Spin-on-Glass Surface Modification,” Biosensors and Bioelectronics, 2004, 20, 83-90.

36. “Photopolymerized Micro-tips for Sample Preparation in Proteomics Analysis” Electrophoresis, 2004, 21-22, 3840-2847.

37. A convenient method to extract MALDI-MS spectra from phosphate-containing peptide mixtures” Proteomics, 2004, 4, 1935-1938.

38. “Microchip Electrophoresis with Hydrodynamic Injection and Waste-removing Function for Quantitative Analysis” J. Chromatogr. A., 2004, 1051, 69-74.

39. Chip-based Microfluidic Devices Coupled with Electrospray Ionization-Mass Spectrometry” Electrophoresis.  Accepted for publication.

40. “Beyond Quantitative Proteomics: Signal Enhancement of the a1 ion as a Mass Tag for Peptide Sequencing Using Dimethyl Labeling” J. Proteome Res.  In Press.

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