莊偉哲教授(特聘)

  • Prof. W.J. Chuang

    分機: 5515 (研究室) 5518 (實驗室); Email

    美國佛羅里達州立大學博士 (1991)

    酵素學、蛋白質化學、儀器分析
    酵素反應機構、結構與設計之研究

    研究方向：

    應用蛋白質生物技術、酵素學、結構生物學與生物資訊進行去結合蛋白與轉錄因子之藥物設計

    研究興趣：

    蛋白質功能與結構的關係之研究、蛋白質生物科技、藥物設計

    研究主題：

  • 去結合蛋白運用於血小板凝聚與癌細胞轉移之研究

  • 三環毒素與其結合受体之研究

  • 翼狀螺旋轉錄因子與其致病突變蛋白之研究

  • A群鏈球菌的致命因子熱原性外毒素B之研究

  • 中草藥有療效成份之分離（鯊魚軟骨、冬蟲夏草、巴西洋菇）

    • 莊老師自己的 Home Page

    Selected publications:

  • Lee, W.-T., Chang, C.-W., Chuang, W.-J., Lin, Y.-S., Wu, J.-J., Lu, C.-C., Tsai, P.-J., and Lin, M. T. (2010) “SPE B-induced Bax expression is through p53 signal pathway in A549 cell”, J. Biomed. Sci., accepted.

  • Tsou CC, Chiang-Ni C, Lin YS, Chuang WJ, Lin MT, Liu CC, Wu JJ. (2010) Oxidative stress and metal ions regulate a ferritin-like gene, dpr, in Streptococcus pyogenes. Int J Med Microbiol. 2009 Oct 28. [Epub ahead of print] [Abstract]

  • Chang CW, Tsai WH, Chuang WJ, Lin YS, Wu JJ, Liu CC, Tsai PJ, Lin MT. (2009) Procaspase 8 and Bax are up-regulated by distinct pathways in Streptococcal pyrogenic exotoxin B-induced apoptosis. J Biol Chem. 2009 Nov 27;284(48):33195-205. Epub 2009 Sep 30. [Abstract]

  • Chang CW, Wu SY, Chuang WJ, Lin YS, Wu JJ, Liu CC, Tsai PJ, Lin MT. (2009) The IL-8 production by Streptococcal pyrogenic exotoxin B. Exp Biol Med (Maywood). 2009 Nov;234(11):1316-26. [Abstract]

  • Chiang-Ni C, Zheng PX, Ho YR, Wu HM, Chuang WJ, Lin YS, Lin MT, Liu CC, Wu JJ. (2009) emm1/sequence type 28 strains of group A streptococci that express covR at early stationary phase are associated with increased growth and earlier SpeB secretion. J Clin Microbiol. 2009 Oct;47(10):3161-9. Epub 2009 Aug 26. [Abstract]

  • Su YF, Wang SM, Lin YL, Chuang WJ, Lin YS, Wu JJ, Lin MT, Liu CC. (2009) Changing epidemiology of Streptococcus pyogenes emm types and associated invasive and noninvasive infections in Southern Taiwan. J Clin Microbiol. 2009 Aug;47(8):2658-61. Epub 2009 Jun 10. [Abstract]

  • Chen CY, Shiu JH, Hsieh YH, Liu YC, Chen YC, Chen YC, Jeng WY, Tang MJ, Lo SJ, Chuang WJ. (2009) Effect of D to E mutation of the RGD motif in rhodostomin on its activity, structure, and dynamics: Importance of the interactions between the D residue and integrin. Proteins. 2009 Sep;76(4):808-21. [2009 Feb 2. Epub ahead of print] [Abstract]

  • Wang CC, Houng HC, Chen CL, Wang PJ, Kuo CF, Lin YS, Wu JJ, Lin MT, Liu CC, Huang W, Chuang WJ. (2009) Solution structure and backbone dynamics of streptopain: insight into diverse substrate specificity. J Biol Chem. 2009 Apr 17;284(16):10957-67. Epub 2009 Feb 23. [Abstract]

  • Chiang-Ni C, Tsou CC, Lin YS, Chuang WJ, Lin MT, Liu CC, Wu JJ. (2008) The transcriptional terminator sequences downstream of the covR gene terminate covR/S operon transcription to generate covR monocistronic transcripts in Streptococcus pyogenes. Gene. 2008 Dec 31;427(1-2):99-103. Epub 2008 Sep 11. [Abstract]

  • Lin HC, Wang SM, Lin YL, Lin YS, Wu JJ, Chuang WJ, Lin MT, Liu CC. (2008) Group A streptococcal infection caused by emm1 strains among children in southern Taiwan. Eur J Clin Microbiol Infect Dis. 2008 Dec;27(12):1253-6. Epub 2008 Jul 3. [Abstract]

  • Tsou CC, Chiang-Ni C, Lin YS, Chuang WJ, Lin MT, Liu CC, Wu JJ. (2008) An iron-binding protein, Dpr, decreases hydrogen peroxide stress and protects Streptococcus pyogenes against multiple stresses. Infect Immun. 2008 Sep;76(9):4038-45. Epub 2008 Jun 9. [Abstract]

  • Wang SM, Lu IH, Lin YL, Lin YS, Wu JJ, Chuang WJ, Lin MT, Liu CC. (2008) The severity of Streptococcus pyogenes infections in children is significantly associated with plasma levels of inflammatory cytokines. Diagn Microbiol Infect Dis. 2008 Jun;61(2):165-9. Epub 2008 Mar 4. [Abstract]

  • Hsu JF, Chuang WJ, Shiesh SC, Lin YS, Liu CC, Wang CC, Fu TF, Tsai JH, Tsai WL, Huang YJ, Hsieh YH, Wu JJ, Lin MT, Huang W. (2008) Streptococcal pyrogenic exotoxin B cleaves human S-adenosylhomocysteine hydrolase and induces hypermethioninemia. J Infect Dis. 2008 Aug 1;198(3):367-74. [Abstract]

  • Tsai WH, Chang CW, Lin YS, Chuang WJ, Wu JJ, Liu CC, Tsai PJ, Lin MT. (2008) Streptococcal pyrogenic exotoxin B-induced apoptosis in A549 cells is mediated through alpha(v)beta(3) integrin and Fas. Infect Immun. 2008 Apr;76(4):1349-57. Epub 2008 Jan 28. [Abstract]

  • Kuo CF, Lin YS, Chuang WJ, Wu JJ, Tsao N. (2008) Degradation of complement 3 by streptococcal pyrogenic exotoxin B inhibits complement activation and neutrophil opsonophagocytosis. Infect Immun. 2008 Mar;76(3):1163-9. Epub 2008 Jan 3. [Abstract]

  • Su CC, Lin YP, Cheng YJ, Huang JY, Chuang WJ, Shan YS, Yang BC. (2007) Phosphatidylinositol 3-kinase/Akt activation by integrin-tumor matrix interaction suppresses Fas-mediated apoptosis in T cells. J Immunol. 2007 Oct 1;179(7):4589-97. [Abstract]

  • Luo YH, Kuo CF, Huang KJ, Wu JJ, Lei HY, Lin MT, Chuang WJ, Liu CC, Lin CF, Lin YS. (2007) Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis. Kidney Int. 2007 Sep;72(6):716-24. Epub 2007 Jul 18. [Abstract]

  • Chang CW, Tsai WH, Chuang WJ, Lin YS, Wu JJ, Liu CC, Tsai PJ, Lin MT. (2007) The fate of SPE B after internalization and its implication in SPEB-induced apoptosis. J Biomed Sci. 2007 May;14(3):419-27. Epub 2007 Mar 23. [Abstract]

  • Wang CC, Chen JH, Lai WC, Chuang WJ. (2007) 2DCSi: identification of protein secondary structure and redox state using 2D cluster analysis of NMR chemical shifts. J Biomol NMR. 2007 May;38(1):57-63. Epub 2007 Feb 27. [Abstract]

  • Anangi, R., Chen, C.-Y., Cheng, C.-H., Chen, Y.-C., Chen, C.-C., Chu, Y.-P., Chang, C.-H., and Chuang, W.-J. (2007) "Expression of Snake Venom Toxins in Pichia pastoris" Toxin Reviews , 26, 169-187.

  • Kuo CF, Chen CC, Lin CF, Jan MS, Huang RY, Luo YH, Chuang WJ, Sheu CC, Lin YS. (2006) Abrogation of streptococcal pyrogenic exotoxin B-mediated suppression of phagocytosis in U937 cells by Cordyceps sinensis mycelium via production of cytokines. Food Chem Toxicol. 2007 Feb;45(2):278-85. Epub 2006 Sep 1.[Abstract]

  • Lu TJ, Lai WY, Huang CY, Hsieh WJ, Yu JS, Hsieh YJ, Chang WT, Leu TH, Chang WC, Chuang WJ, Tang MJ, Chen TY, Lu TL, Lai MD. (2006) Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein-tyrosine phosphatase-PEST. J Biol Chem. 2006 Dec 15;281(50):38405-17. Epub 2006 Oct 17. [Abstract]

  • Nooh MM, Aziz RK, Kotb M, Eroshkin A, Chuang WJ, Proft T, Kansal R. (2006) Streptococcal Mitogenic Exotoxin, SmeZ, Is the Most Susceptible M1T1 Streptococcal Superantigen to Degradation by the Streptococcal Cysteine Protease, SpeB. J Biol Chem. 2006 Nov 17;281(46):35281-8. Epub 2006 Sep 15. [Abstract]

  • Tsai KL, Huang CY, Chang CH, Sun YJ, Chuang WJ, Hsiao CD. (2006) Crystal structure of the human FOXK1a-DNA complex and its implications on the diverse binding specificity of winged helix/forkhead proteins. J Biol Chem. 2006 Jun 23;281(25):17400-9. Epub 2006 Apr 18. [Abstract]

  • Tang CH, Yang RS, Huang TH, Lu DY, Chuang WJ, Huang TF, Fu WM. (2006) Ultrasound stimulates cyclooxygenase-2 expression and increases bone formation through integrin, focal adhesion kinase, phosphatidylinositol 3-kinase, and Akt pathway in osteoblasts. Mol Pharmacol. 2006 Jun;69(6):2047-57. Epub 2006 Mar 15. [Abstract]

  • Tsao N, Tsai WH, Lin YS, Chuang WJ, Wang CH, Kuo CF. (2006) Streptococcal pyrogenic exotoxin B cleaves properdin and inhibits complement-mediated opsonophagocytosis. Biochem Biophys Res Commun. 2006 Jan 20;339(3):779-84. Epub 2005 Nov 22. [Abstract]

  • Wang CC, Chen JH, Yin SH, Chuang WJ. (2006) redicting the redox state and secondary structure of cysteine residues in proteins using NMR chemical shifts. Proteins. 2006 Jan 27;63(1):219-226 [Epub ahead of print] [Abstract]

  • Chen CY, Cheng CH, Chen YC, Lee JC, Chou SH, Huang W, Chuang WJ. (2006) Preparation of amino-acid-type selective isotope labeling of protein expressed in Pichia pastoris. Proteins. 2006 Jan 1;62(1):279-87. [Abstract]

  • Lin YT, Tang CH, Chuang WJ, Wang SM, Huang TF, Fu WM. (2005) Inhibition of adipogenesis by RGD-dependent disintegrin. Biochem Pharmacol. 2005 Nov 15;70(10):1469-78. Epub 2005 Sep 28. [Abstract]

  • Chiang-Ni C, Wang CH, Tsai PJ, Chuang WJ, Lin YS, Lin MT, Liu CC, Wu JJ. (2005) Streptococcal pyrogenic exotoxin B causes mitochondria damage to polymorphonuclear cells preventing phagocytosis of Group A streptococcus. Med Microbiol Immunol (Berl). 2005 Jul 30;:1-9 [Epub ahead of print] [Abstract]

  • Hsieh HC, Hsieh YH, Huang YH, Shen FC, Tsai HN, Tsai JH, Lai YT, Wang YT, Chuang WJ, Huang W. (2005) HHR23A, a human homolog of Saccharomyces cerevisiae Rad23, regulates xeroderma pigmentosum C protein and is required for nucleotide excision repair. Biochem Biophys Res Commun. 2005 Sep 16;335(1):181-7. [Abstract]

  • Kuo CF, Chen CC, Luo YH, Huang RY, Chuang WJ, Sheu CC, Lin YS. (2005) Cordyceps sinensis mycelium protects mice from group A streptococcal infection. J Med Microbiol. 2005 Aug;54(Pt 8):795-802. [Abstract]

  • Wang CH, Lin CY, Luo YH, Tsai PJ, Lin YS, Lin MT, Chuang WJ, Liu CC, Wu JJ. (2005) Effects of oligopeptide permease in group a streptococcal infection. Infect Immun. 2005 May;73(5):2881-90. [Abstract]

  • Yang RS, Tang CH, Chuang WJ, Huang TH, Peng HC, Huang TF, Fu WM. (2005) Inhibition of tumor formation by snake venom disintegrin. Toxicon. 2005 Apr;45(5):661-9. [Abstract]

  • Su SJ, Yeh TM, Chuang WJ, Ho CL, Chang KL, Cheng HL, Liu HS, Cheng HL, Hsu PY, Chow NH. (2005) The novel targets for anti-angiogenesis of genistein on human cancer cells. Biochem Pharmacol. 2005 Jan 15;69(2):307-18. Epub 2004 Nov 19. [Abstract]

  • Tsai WH, Chang CW, Chuang WJ, Lin YS, Wu JJ, Liu CC, Chang WT, Lin MT. (2004) Streptococcal pyrogenic exotoxin B-induced apoptosis in a549 cells is mediated by a receptor- and mitochondrion-dependent pathway. Infect Immun. 2004 Dec;72(12):7055-62. [Abstract]

  • Shiu JH, Chen CY, Chang LS, Chen YC, Chen YC, Lo YH, Liu YC, Chuang WJ. (2004) Solution structure of gamma-bungarotoxin: The functional significance of amino acid residues flanking the RGD motif in integrin binding. Proteins. 2004 Aug 18;57(4):839-849 [Epub ahead of print] [Abstract]

  • Jeng WY, Tsai YH, Chuang WJ. (2004) The catalase activity of Nalpha-acetyl-microperoxidase-8. J Pept Res. 2004 Sep;64(3):104-9. [Abstract]

  • Kuo CF, Luo YH, Lin HY, Huang KJ, Wu JJ, Lei HY, Lin MT, Chuang WJ, Liu CC, Jin YT, Lin YS. (2004) Histopathologic changes in kidney and liver correlate with streptococcal pyrogenic exotoxin B production in the mouse model of group A streptococcal infection. Microb Pathog. 2004 May;36(5):273-85. [Abstract]

  • Yan JJ, Liu CC, Ko WC, Hsu SY, Wu HM, Lin YS, Lin MT, Chuang WJ, Wu JJ. (2003) Molecular analysis of group A streptococcal isolates associated with scarlet fever in southern Taiwan between 1993 and 2002. J Clin Microbiol. 2003 Oct;41(10):4858-61. [Abstract]

  • Chen, C.-Y., Kuo, C.-F., Lin, T.-S., Wu, J.-J., Lin. M.-T., Liu, C.-C. and Chuang, W.-J. (2003) Maturation Processing and Characterization of Streptopain, J Biol Chem 2003 May 9;278(19):17336-43. [Abstract]

  • Luo, S.-C., Chen, C.-Y., Lin. T.-S., Jeng, W.-Y., and Chuang, W.-J. (2003) Backbone (1)H, (15)N and (13)C resonance assignments of the 28 kDa mature form of streptopain(*). J Biomol NMR 2003 Feb;25(2):165-6. [Abstract]

  • Chuang, W.-J., Wu, C.-H., Huang, H.-N., Chen, S.-H., Hsiao, G., Lin, C.-H., and Sheu, J.-R. (2003). Comparison of the binding character of triflavin on resting and activated alpha(IIb)beta(3) integrin in human platelets by electron microscopy. Thromb Res 2003 Jan 1;109(1):37-46. [Abstract]

  • Kansal, R. G., Nizet, V., Jeng, A., Chuang, W.-J. , and Kotb, M. (2003) Selective modulation of superantigen-induced responses by streptococcal cysteine protease. J Infect Dis 2003 Feb 1;187(3):398-407. [Abstract]

  • Jeng, W.-Y. Chen, C.-Y., Chang, H. C., and Chuang, W.-J. (2002) Expression and characterization of recombinant human cytochrome c in E. coli. J Bioenerg Biomembr 2002 Dec;34(6):423-31. [Abstract]

  • Chuang, W.-J., Yeh, I-Ju, Hsieh, Y.-H. Chen, S.-W., Liu, P.-P., Jeng, W.-Y. (2002) 1H, 15N and 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor (Foxk1). J Biomol NMR 2002 Sep;24(1):75-6. [Abstract]

  • Liu, P.-P., Li, C., Hsieh, Y.-H. Chen, S.-W., Chen, S.-H., and Chuang, W.-J. (2002) Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a). Proteins 2002 Dec 1;49(4):543-53. [Abstract]

  • Huang, B.-M., Ju, S.-Y., Wu, C.-S., Chuang, W.-J., Sheu, C.-C., Leu, S.-F. (2001) Cordyceps sinensis and its fractions stimulate MA-10 mouse Leydig tumor cell steroidogenesis. J Androl 2001 Sep-Oct;22(5):831-7. [Abstract]

  • Wu, D.-H., Shi, G.-Y., Chuang, W.-J., Hsu, J.-M., Young, K.-C., Chang, C.-W., and Wu, H.-L (2001) Coiled coil region of streptokinase gamma-domain is essential for plasminogen activation. J Biol Chem 2001 May 4;276(18):15025-33. [Abstract]

  • Guo, R.-T., Chou, L.-J., Chen, Y.-C., Chen, C.-y., Pari, K., Jen, C. J., Lo, S. J., Huang, S.-L. Lee, C-Y, Chang, T.-W., and Chuang, W.-J. Expression in Pichia pastoris and characterization by circular dichroism and NMR of rhodostomin. Proteins 2001 Jun 1;43(4):499-508. [Abstract]

  • Jiang S.-T., Chiu S.-J., Chen H.-C., Chuang, W.-J., and Tang, M.-J. (2001) The Role of Integrin a3b1 in Tubulogenesis of MDCK Cells. Kidney Int 2001 May;59(5):1770-8. [Abstract]

  • Jiang S.-T., Chuang, W.-J., and Tang, M.-J. (2000) Role of fibronectin deposition in branching morphogenesis of Madin-Darby canine kidney cells. Kidney Int 2000 May;57(5):1860-7. [Abstract]

  • Jiang S.-T., Chiang, H.-C., Cheng, M.-H., Yang, T.-P., Chuang, W.-J., and Tang, M.-J. (1999) Role of fibronectin deposition in cystogenesis of Madin-Darby canine kidney cells. Kidney Int 1999 Jul;56(1):92-103. [Abstract]

  • Chuang, W.-J., Chang, Y.-D., and Wen-Yih Jeng (1999) Kinetic and structural studies of N-acetyl-microperoxidase-5 and -microperoxidase-8. JOURNAL OF INORGANIC BIOCHEMISTRY 75 (2): 93-97 JUN 15 1999. [Abstract(cited from Web of Science)]

  • Sheu, J.-R., Chang, C.-F., Tsai, M.-L., and Chuang, W.-J. (1998) Effect of U-995, a potent shark cartilage-derived angiogenesis inhibitor, on anti-angiogenesis and anti-tumor activities. Anticancer Res 1998 Nov-Dec;18(6A):4435-42. [Abstract]

  • Shieh, B., Su, I.-J., Chuang, W.-J., and Li, C. (1998) Dye-induced denaturation of DNA dissolved in water. Biotechniques 1998 Jan;24(1):60-2, 64.

  • Chen, H.-I., Wu, Y.-I, Hsieh, Y.-L., Shi, G.-Y., Jiang, M.-J., Chang, W.-C., Chuang, W.-J., Kan, W.-M. Tang, M.-J., and Jen, C. J. (1997) Perturbation of platelet adhesion to endothelial cells by plasminogen activation in vitro. Thromb Haemost 1997 Aug;78(2):934-8. [Abstract]

  • Young, K.-C., Shi, G.-Y., Chang, Y.-F., Chang, B.-I., Chang, L.-C., Lai, M.-D., Chuang, W.-J., and Wu, H.-L. (1995) Interaction of streptokinase and plasminogen. Studied with truncated streptokinase peptides. J Biol Chem 1995 Dec 8;270(49):29601-6. [Abstract]

  • Chuang, W.-J., Abeygunawardana, C., Gittis, A. G., Pedersen, P. L., and Mildvan, A. S. (1995) Solution structure and function in trifluoroethanol of PP-50, an ATP-binding peptide from F1ATPase. Arch Biochem Biophys 1995 May 10;319(1):110-22. [Abstract]

  • Chuang, W.-J., Gittis, A. G., and Mildvan, A. S. (1994) Magnetic resonance studies of the binding of oligonucleotide substrates to mutants of staphylococcal nuclease. Proteins 1994 Jan;18(1):68-80. [Abstract]

  • Chuang, W.-J., Weber, D. J., Gittis, A. J., and Mildvan, A. S. (1993) Mutational tests of the NMR-docked structure of the staphylococcal nuclease-metal-3',5'-pdTp complex. Proteins 1993 Sep;17(1):36-48. [Abstract]

  • Chuang, W.-J., Abeygunawardana, C., Pedersen, P. L., and Mildvan, A. S. (1992) Two-dimensional NMR, circular dichroism, and fluorescence studies of PP-50, a synthetic ATP-binding peptide from the beta-subunit of mitochondrial ATP synthase. Biochemistry 1992 Sep 1;31(34):7915-21. [Abstract]

  • Chuang, W.-J., and Van Wart, H. E. (1992) Resonance Raman spectra of horseradish peroxidase and bovine liver catalase compound I species. Evidence for predominant 2A2u pi-cation radical ground state configurations. J Biol Chem 1992 Jul 5;267(19):13293-301. [Abstract]

  • Chuang, W.-J., Heldt, J., and Van Wart, H. E. (1989) Resonance Raman spectra of bovine liver catalase compound II. Similarity of the heme environment to horseradish peroxidase compound II. J Biol Chem 1989 Aug 25;264(24):14209-15. [Abstract]

  • Chuang, W.-J., Johnson, S., and Van Wart H. E. (1988) Resonance Raman spectra of bovine liver catalase: enhancement of proximal tyrosinate vibrations. J Inorg Biochem 1988 Nov;34(3):201-19. [Abstract]

    Return to English Main Page
     Return to NCKU Chinese Main Page of Biochem 回到生化所...

    Send comments or Suggestion to this email box.

    Updated: 05 Feb. 2010