施桂月教授
﹝兼所長﹞

  • Prof. G.Y. Shi

    分機: 5540 (研究室) 5525 (實驗室); Email

    美國俄亥俄州立大學博士

    生物能量學、中間代謝、臨床生化學

    研究方向：

    凝血，血栓溶解及抗癌相關蛋白之研究、基因轉殖、基因調控、訊息傳遞

    研究興趣：

  • 人類凝血(酉+每)調節素（Thrombomodulin）重組蛋白片段之研究。利用基因重組技術與酵母菌 pichia 蛋白質表現系統，來大量製造具有功能的凝血(酉+每)調節素等珍貴醫藥製品，並在細胞及動物模式中研究各功能定義區之抗發炎與刺激血管新生等功能與應用。此外，亦以 RNAi 為工具研究特定基因片段之功能。另一方面，則以基因轉殖小鼠模式來研究 TM 的功能。

  • 以 Apo-E 基因缺陷小鼠為模式，研究動脈粥狀硬化(atherosclerosis)之機轉與藥物之療效。並以微矩陣基因分析及定量 PCR 研究抗發炎藥物對血管細胞基因表現的影響。

    • Selected publications:

  • Li YH, Chung HC, Luo CY, Chao TH, Shyu KG, Shi GY, Wu HL. (2010) Thrombomodulin is upregulated in cardiomyocytes during cardiac hypertrophy and prevents the progression of contractile dysfunction. J Card Fail. 2010 Dec;16(12):980-90. [Abstract]

  • Kao YC, Wu LW, Shi CS, Chu CH, Huang CW, Kuo CP, Sheu HM, Shi GY, Wu HL. (2010) Downregulation of thrombomodulin, a novel target of Snail, induces tumorigenesis through epithelial-mesenchymal transition. Mol Cell Biol. 2010 Oct;30(20):4767-85. Epub 2010 Aug 16. [Abstract]

  • Lin CN, Kao CY, Miao CH, Hamaguchi N, Wu HL, Shi GY, Liu YL, High KA, Lin SW. (2010) Generation of a novel factor IX with augmented clotting activities in vitro and in vivo. J Thromb Haemost. 2010 Aug;8(8):1773-83. doi: 10.1111/j.1538-7836.2010.03913.x. Epub 2010 May 21. [Abstract]

  • Kao CY, Lin CN, Yu IS, Tao MH, Wu HL, Shi GY, Yang YL, Kao JT, Lin SW. (2010) FIX-Triple, a gain-of-function factor IX variant, improves haemostasis in mouse models without increased risk of thrombosis. Thromb Haemost. 2010 Aug 2;104(2):355-65. Epub 2010 Jun 10. [Abstract]

  • Chang PC, Wu HL, Lin HC, Wang KC, Shi GY. (2010) Human plasminogen kringle 1-5 reduces atherosclerosis and neointima formation in mice by suppressing the inflammatory signaling pathway. J Thromb Haemost. 2010 Jan;8(1):194-201. Epub 2009 Oct 30. [Abstract]

  • Chang PC, Wu HL, Lin HC, Wang KC, Shi GY. (2009) Human plasminogen kringle 1-5 reduces atherosclerosis and neointima formation in mice by suppressing the inflammatory signaling pathway. J Thromb Haemost. 2009 Oct 30. [Epub ahead of print] [Abstract]

  • Huang YL, Wu CM, Shi GY, Wu GC, Lee H, Jiang MJ, Wu HL, Yang HY. (2009) Nestin serves as a prosurvival determinant that is linked to the cytoprotective effect of epidermal growth factor in rat vascular smooth muscle cells. J Biochem. 2009 Sep;146(3):307-15. Epub 2009 May 18. [Abstract]

  • Li YH, Shi GY, Wu HL. (2009) Thrombomodulin in the treatment of atherothrombotic diseases. Front Biosci (Schol Ed). 2009 Jun 1;1:33-8. [Abstract]

  • Huang YL, Shi GY, Lee H, Jiang MJ, Huang BM, Wu HL, Yang HY. (2009) Thrombin induces nestin expression via the transactivation of EGFR signalings in rat vascular smooth muscle cells. Cell Signal. 2009 Jun;21(6):954-68. Epub 2009 Feb 24. [Abstract]

  • Liu SL, Li YH, Shi GY, Tang SH, Jiang SJ, Huang CW, Liu PY, Hong JS, Wu HL. (2009) Dextromethorphan reduces oxidative stress and inhibits atherosclerosis and neointima formation in mice. Cardiovasc Res. 2009 Apr 1;82(1):161-9. Epub 2009 Feb 3. [Abstract]

  • Lo IC, Lin TM, Chou LH, Liu SL, Wu LW, Shi GY, Wu HL, Jiang MJ. (2009) Ets-1 mediates platelet-derived growth factor-BB-induced thrombomodulin expression in human vascular smooth muscle cells. Cardiovasc Res. 2009 Mar 1;81(4):771-9. Epub 2008 Dec 17. [Abstract]

  • Kao TT, Wang KC, Chang WN, Lin CY, Chen BH, Wu HL, Shi GY, Tsai JN, Fu TF. (2009) Characterization and comparative studies of zebrafish and human recombinant dihydrofolate reductases--inhibition by folic acid and polyphenols. Drug Metab Dispos. 2008 Mar;36(3):508-16. Epub 2007 Dec 3. [Abstract]

  • Kao TT, Chang WN, Wu HL, Shi GY, Fu TF. (2009) Recombinant zebrafish {gamma}-glutamyl hydrolase exhibits properties and catalytic activities comparable with those of mammalian enzyme. Drug Metab Dispos. 2009 Feb;37(2):302-9. Epub 2008 Nov 12. [Abstract]

  • Huang YL, Shi GY, Jiang MJ, Lee H, Chou YW, Wu HL, Yang HY. (2008) Epidermal growth factor up-regulates the expression of nestin through the Ras-Raf-ERK signaling axis in rat vascular smooth muscle cells. Biochem Biophys Res Commun. 2008 Dec 12;377(2):361-6. Epub 2008 Oct 11. [Abstract]

  • Liu PY, Lin CC, Tsai WC, Li YH, Lin LJ, Shi GY, Hong JS, Chen JH, Wu HL. (2008) Treatment with dextromethorphan improves endothelial function, inflammation and oxidative stress in male heavy smokers. J Thromb Haemost. 2008 Oct;6(10):1685-92. Epub 2008 Jul 18. [Abstract]

  • Shi CS, Shi GY, Hsiao SM, Kao YC, Kuo KL, Ma CY, Kuo CH, Chang BI, Chang CF, Lin CH, Wong CH, Wu HL. (2008) Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood. 2008 Nov 1;112(9):3661-70. Epub 2008 Aug 18. [Abstract]

  • Chang PC, Chang YJ, Wu HL, Chang CW, Lin CI, Wang WC, Shi GY. (2008) Mutation of human plasminogen kringle 1-5 enhances anti-angiogenic action via increased interaction with integrin alpha(v)beta(3). Thromb Haemost. 2008 Apr;99(4):729-38. [Abstract]

  • Kao TT, Wang KC, Chang WN, Lin CY, Chen BH, Wu HL, Shi GY, Tsai JN, Fu TF. (2008) Characterization and comparative studies of zebrafish and human recombinant dihydrofolate reductases--inhibition by folic acid and polyphenols. Drug Metab Dispos. 2008 Mar;36(3):508-16. Epub 2007 Dec 3. [Abstract]

  • Wu HL, Lin CI, Huang YL, Chen PS, Kuo CH, Chen MS, Wu GC, Shi GY, Yang HY, Lee H. (2008) Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells. Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells. Biochem Biophys Res Commun. 2008 Feb 29;367(1):162-8. Epub 2007 Dec 31. [Abstract]

  • Li YH, Hsieh CY, Wang DL, Chung HC, Liu SL, Chao TH, Shi GY, Wu HL. (2007) Remodeling of carotid arteries is associated with increased expression of thrombomodulin in a mouse transverse aortic constriction model. Thromb Haemost. 2007 Apr;97(4):658-64. [Abstract]

  • Liu SL, Li YH, Shi GY, Jiang MJ, Chang JH, Wu HL. (2007) The effect of statin on the aortic gene expression profiling. Int J Cardiol. 2007 Jan 2;114(1):71-7. Epub 2006 May 5. [Abstract]

  • Shi CS, Huang HC, Wu HL, Kuo CH, Chang BI, Shiao MS, Shi GY. (2007) Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells. Thromb Res. 2006 Jul 14; [Epub ahead of print] [Abstract]

  • Shi CS, Huang HC, Wu HL, Kuo CH, Chang BI, Shiao MS, Shi GY. (2007) Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells. Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells. Thromb Res. 2007;119(6):769-75. Epub 2006 Jul 17. [Abstract]

  • Liu SL, Li YH, Shi GY, Chen YH, Huang CW, Hong JS, Wu HL. (2006) A novel inhibitory effect of naloxone on macrophage activation and atherosclerosis formation in mice. J Am Coll Cardiol. 2006 Nov 7;48(9):1871-9. Epub 2006 Oct 17. [Abstract]

  • Liu PY, Li YH, Chan SH, Lin LJ, Wu HL, Shi GY, Chen JH. (2006) Genotype-phenotype association of matrix metalloproteinase-3 polymorphism and its synergistic effect with smoking on the occurrence of acute coronary syndrome. Am J Cardiol. 2006 Oct 15;98(8):1012-7. Epub 2006 Aug 22. [Abstract]

  • Liu PY, Li YH, Wu HL, Chao TH, Tsai LM, Lin LJ, Shi GY, Chen JH. (2006) Platelet-activating factor-acetylhydrolase A379V (exon 11) gene polymorphism is an independent and functional risk factor for premature myocardial infarction. J Thromb Haemost. 2006 May;4(5):1023-8. [Abstract]

  • Li YH, Shi GY, Wu HL. (2006) The role of thrombomodulin in atherosclerosis: from bench to bedside. Cardiovasc Hematol Agents Med Chem. 2006 Apr;4(2):183-7. Review. [Abstract]

  • Li YH, Liu SL, Shi GY, Tseng GH, Liu PY, Wu HL. (2006) Thrombomodulin plays an important role in arterial remodeling and neointima formation in mouse carotid ligation model. Thromb Haemost. 2006 Jan;95(1):128-33. [Abstract]

  • Shi CS, Shi GY, Chang YS, Han HS, Kuo CH, Liu C, Huang HC, Chang YJ, Chen PS, Wu HL. (2005) Evidence of Human Thrombomodulin Domain as a Novel Angiogenic Factor. Circulation. 2005 Apr 5;111(13):1627-36. Epub 2005 Mar 28. [Abstract]

  • Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. (2004) Inhibition of bovine herpesvirus-4 replication by arsenite through downregulation of the extracellular signal-regulated kinase signaling pathway. J Biomed Sci. 2004 Jul-Aug;11(4):500-10. [Abstract]

  • Yu IS, Lin SR, Huang CC, Tseng HY, Huang PH, Shi GY, Wu HL, Tang CL, Chu PH, Wang LH, Wu KK, Lin SW. (2004) TXAS-deleted mice exhibit normal thrombopoiesis, defective hemostasis, and resistance to arachidonate-induced death. Blood. 2004 Jul 1;104(1):135-42. Epub 2004 Mar 9. [Abstract]

  • Chao TH, Li YH, Chen JH, Wu HL, Shi GY, Tsai WC, Chen PS, Liu PY. (2004) Relation of thrombomodulin gene polymorphisms to acute myocardial infarction in patients Am J Cardiol. 2004 Jan 15;93(2):204-7. [Abstract]

  • Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. (2004) Inhibition of bovine herpesvirus-4 replication in endothelial cells by arsenite. Antiviral Res. 2004 Sep;63(3):167-75. [Abstract]

  • Chao TH, Li YH, Chen JH, Wu HL, Shi GY, Liu PY, Tsai WC, Guo HR. (2004) The 161TT genotype in the exon 6 of the peroxisome-proliferator-activated receptor gamma gene is associated with premature acute myocardial infarction and increased lipid peroxidation in habitual heavy smokers. Clin Sci (Lond). 2004 Nov;107(5):461-6. [Abstract]

  • Chang YJ, Wu HL, Hsu YC, Hamaguchi N, Shi GY, Shen MC, Lin SW. (2003) Discontinuous residues of factor IX constitute a surface for binding the anti-factor IX monoclonal antibody A-5. Thromb Res. 2003;111(4-5):293-9. [Abstract]

  • Huang HC, Shi GY, Jiang SJ, Shi CS, Wu CM, Yang HY, Wu HL. (2003) Thrombomodulin-mediated cell adhesion: involvement of its lectin-like domain. J Biol Chem. 2003 Nov 21;278(47):46750-9. Epub 2003 Sep 01. [Abstract]

  • Liu PY, Chen JH, Li YH, Wu HL, Shi GY. (2003) Synergistic effect of stromelysin-1 (matrix metallo-proteinase-3) promoter 5A/6A polymorphism with smoking on the onset of young acute myocardial infarction. Thromb Haemost. 2003 Jul;90(1):132-9. [Abstract]

  • Jiang SJ, Lin TM, Wu HL, Han HS, Shi GY. (2002) Decrease of fibrinolytic activity in human endothelial cells by arsenite. Thromb Res 2002 Jan 1;105(1):55-62 [Abstract]

  • Li YH, Chen JH, Tsai WC, Chao TH, Guo HR, Tsai LM, Wu HL, Shi GY. (2002) Synergistic effect of thrombomodulin promoter -33G/A polymorphism and smoking on the onset of acute myocardial infarction. Thromb Haemost 2002 Jan;87(1):86-91[Abstract]

  • Han HS, Yang SL, Yeh HY, Lin JC, Wu HL, Shi GY. (2001) Studies of a novel human thrombomodulin immobilized substrate: surface characterization and anticoagulation activity evaluation. J Biomater Sci Polym Ed 2001;12(10):1075-89 [Abstract]

  • Shieh SJ, Chiu HY, Shi GY, Wu CM, Wang JC, Chen CH, Wu HL. (2001) A novel platelet-rich arterial thrombosis model in rabbits. Simple, reproducible, and dynamic real-time measurement by using double-opposing inverted-sutures model. Thromb Res 2001 Sep 1;103(5):363-76 [Abstract]

  • Li YH, Chen CH, Yeh PS, Lin HJ, Chang BI, Lin JC, Guo HR, Wu HL, Shi GY, Lai ML, Chen JH. (2001) Functional mutation in the promoter region of thrombomodulin gene in relation to carotid atherosclerosis. Atherosclerosis 2001 Feb 15;154(3):713-9 [Abstract]

  • Li YH, Chen JH, Wu HL, Shi GY, Huang HC, Chao TH, Tsai WC, Tsai LM, Guo HR, Wu WS, Chen ZC. (2000) G-33A mutation in the promoter region of thrombomodulin gene and its association with coronary artery disease and plasma soluble thrombomodulin levels. Am J Cardiol 2000 Jan 1;85(1):8-12 [Abstract]

  • Lin TM, Jiang MJ, Eng HL, Shi GY, Lai LC, Huang BJ, Huang KY, Wu HL. (2000) Experimental infection with bovine herpesvirus-4 enhances atherosclerotic process in rabbits. Lab Invest 2000 Jan;80(1):3-11 [Abstract]

  • Lin TM, Shi GY, Jiang SJ, Tsai CF, Hwang BJ, Hsieh CT, Wu HL. (1999) Persistent infection of bovine herpesvirus type 4 in bovine endothelial cell cultures. Vet Microbiol 1999 Oct;70(1-2):41-53 [Abstract]

  • Cao R, Wu HL, Veitonmaki N, Linden P, Farnebo J, Shi GY, Cao Y. (1999) Suppression of angiogenesis and tumor growth by the inhibitor K1-5 generated by plasmin-mediated proteolysis. Proc Natl Acad Sci U S A 1999 May 11;96(10):5728-33 [Abstract]

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    Updated: 24, Dec. 2010