賴明德教授 (特聘)

  • Prof. M.D. Lai

    分機: 5549 (研究室) 5552 (實驗室); Email

    美國貝勒醫學院博士 (1987) 

    分子生物學、腫廇分生學

    研究方向:

    膀胱癌、基因治療、致癌基因、抑癌基因、抗藥性、 p53 、 neu 、肝癌之研究

    研究興趣:

      本實驗室的研究主要在了解致癌基及腫瘤抑制基因如何交互作用而使細胞 產生癌化現象。我們將肝癌或膀胱癌細胞株中的致癌或腫瘤抑制基因加以改變,再觀察 不同基因的突變如何影響細胞在体外之生長及老鼠体內的致癌能力及癌轉移現象。目前 的研究集中在致癌基因 neu 及腫瘤抑制基因 P53 的交互作用及在細胞內的作用機轉。 我們同時也利用 neu DNA 疫苗治療膀胱癌,並研究基因免疫治療法之作用機轉。

    Selected publications:

  • Wang LH, Huang W, Lai MD, Su IJ. (2011) Aberrant cyclin A expression and centrosome overduplication induced by hepatitis B virus pre-S2 mutants and its implication in hepatocarcinogenesis. Carcinogenesis. 2012 Feb;33(2):466-72. Epub 2011 Dec 9. [Abstract]

  • Huang TT, Yen MC, Lin CC, Weng TY, Chen YL, Lin CM, Lai MD. (2011) Skin delivery of short hairpin RNA of indoleamine 2,3 dioxygenase induces antitumor immunity against orthotopic and metastatic liver cancer. Cancer Sci. 2011 Dec;102(12):2214-20. doi: 10.1111/j.1349-7006.2011.02094.x. Epub 2011 Oct 17. [Abstract]

  • Shan YS, Fang JH, Lai MD, Yen MC, Lin PW, Hsu HP, Lin CY, Chen YL. (2011) Establishment of an orthotopic transplantable gastric cancer animal model for studying the immunological effects of new cancer therapeutic modules. Mol Carcinog. 2011 Oct;50(10):739-50. doi: 10.1002/mc.20668. Epub 2010 Aug 24. [Abstract]

  • Lee JW, Liao PC, Young KC, Chang CL, Chen SS, Chang TT, Lai MD, Wang SW. (2011) Identification of hnRNPH1, NF45, and C14orf166 as novel host interacting partners of the mature hepatitis C virus core protein. J Proteome Res. 2011 Oct 7;10(10):4522-34. Epub 2011 Aug 24. [Abstract]

  • Chang YS, Tsai CT, Huangfu CA, Huang WY, Lei HY, Lin CF, Su IJ, Chang WT, Wu PH, Chen YT, Hung JH, Young KC, Lai MD. (2011) ACSL3 and GSK-3β are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells. J Cell Biochem. 2011 Mar;112(3):881-93. doi: 10.1002/jcb.22996. [Abstract]

  • Chen SY, Wu CL, Lai MD, Lin CC, Yo YT, Jou IM, Lee CH, Weng CT, Shiau AL, Wang CR. (2010) Amelioration of Rat Collagen-induced Arthritis through CD4+ T Cells Apoptosis and Synovial Interleukin-17 Reduction by Indoleamine 2,3-dioxygenase Gene Therapy. Hum Gene Ther. 2011 Feb;22(2):145-54. Epub 2011 Jan 11. [Epub ahead of print, 2010 Sep 8.] [Abstract]

  • Liu HS, Hsu PY, Lai MD, Chang HY, Ho CL, Cheng HL, Chen HT, Lin YJ, Wu TJ, Tzai TS, Chow NH. (2010) An unusual function of RON receptor tyrosine kinase as a transcriptional regulator in cooperation with EGFR in human cancer cells. Carcinogenesis. 2010 Aug;31(8):1456-64. Epub 2010 May 24. [Abstract]

  • Shan YS, Fang JH, Lai MD, Yen MC, Lin PW, Hsu HP, Lin CY, Chen YL. (2010) Establishment of an orthotopic transplantable gastric cancer animal model for studying the immunological effects of new cancer therapeutic modules. Mol Carcinog. 2010 Aug 24. [Epub ahead of print] [Abstract]

  • Yang HJ, Yen MC, Lin CC, Lin CM, Chen YL, Weng TY, Huang TT, Wu CL, Lai MD. (2010) A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity. Exp Biol Med (Maywood). 2010 Jul;235(7):869-76. [Abstract]

  • Hsu HP, Shan YS, Lai MD, Lin PW. (2010) Osteopontin-positive infiltrating tumor-associated macrophages in bulky ampullary cancer predict survival. Cancer Biol Ther. 2010 Jul 15;10(2):144-54. Epub 2010 Jul 26. [Abstract]

  • Hung JH, Yan CW, Su IJ, Wang HC, Lei HY, Lin WC, Chang WT, Huang W, Lu TJ, Lai MD. (2010) Hepatitis B virus surface antigen interacts with acid alpha-glucosidase and alters glycogen metabolism. Hepatol Res. 2010 Jun;40(6):633-40. [Abstract]

  • Hsu HP, Shan YS, Jin YT, Lai MD, Lin PW. (2010) Loss of E-cadherin and beta-catenin is correlated with poor prognosis of ampullary neoplasms. J Surg Oncol. 2010 Apr 1;101(5):356-62. [Abstract]

  • Lai MD, Chen CS, Yang CR, Yuan SY, Tsai JJ, Tu CF, Wang CC, Yen MC, Lin CC. (2010) An HDAC inhibitor enhances the antitumor activity of a CMV promoter-driven DNA vaccine. Cancer Gene Ther. 2010 Mar;17(3):203-11. Epub 2009 Oct 23. [Abstract]

  • Ko TP, Jeng WY, Liu CI, Lai MD, Wu CL, Chang WJ, Shr HL, Lu TJ, Wang AH. (2010) Structures of human MST3 kinase in complex with adenine, ADP and Mn2+. Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):145-54. Epub 2010 Jan 22. [Abstract]

  • Chuang JY, Wu CH, Lai MD, Chang WC, Hung JJ. (2009) Overexpression of Sp1 leads to p53-dependent apoptosis in cancer cells. Int J Cancer. 2009 Nov 1;125(9):2066-76. [Abstract]

  • Lai MD, Chen CS, Yang CR, Yuan SY, Tsai JJ, Tu CF, Wang CC, Yen MC, Lin CC. (2009) An HDAC inhibitor enhances the antitumor activity of a CMV promoter-driven DNA vaccine. Cancer Gene Ther. 2009 Oct 23. [Epub ahead of print] [Abstract]

  • Yen MC, Lin CC, Chen YL, Huang SS, Yang HJ, Chang CP, Lei HY, Lai MD. (2009) A novel cancer therapy by skin delivery of indoleamine 2,3-dioxygenase siRNA. Clin Cancer Res. 2009 Jan 15;15(2):641-9. [Abstract]

  • Lai MD, Yen MC, Lin CM, Tu CF, Wang CC, Lin PS, Yang HJ, Lin CC. (2009) The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model. Genet Vaccines Ther. 2009 Jan 30;7:2. [Abstract]

  • Chen YL, Fang JH, Lai MD, Shan YS. (2008) Depletion of CD4(+)CD25(+) regulatory T cells can promote local immunity to suppress tumor growth in benzo[a]pyrene-induced forestomach carcinoma. World J Gastroenterol. 2008 Oct 14;14(38):5797-809. [Abstract]

  • Wei S, Yang HC, Chuang HC, Yang J, Kulp SK, Lu PJ, Lai MD, Chen CS. (2008) A novel mechanism by which thiazolidinediones facilitate the proteasomal degradation of cyclin D1 in cancer cells. J Biol Chem. 2008 Sep 26;283(39):26759-70. Epub 2008 Jul 23. [Abstract]

  • Chang WW, Su IJ, Lai MD, Chang WT, Huang W, Lei HY. (2008) Suppression of p38 mitogen-activated protein kinase inhibits hepatitis B virus replication in human hepatoma cell: the antiviral role of nitric oxide. J Viral Hepat. 2008;15:490-7. [Abstract]

  • Lin CC, Yen MC, Lin CM, Huang SS, Yang HJ, Chow NH, Lai MD. Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer. J Gene Med. 2008 Jun;10(6):679-89. [Abstract]

  • Lin CC, Yang HJ, Tu CF, Lai MD. (2008) The opposing effects of lipopolysaccharide on the antitumor therapeutic efficacy of DNA vaccine. DNA Cell Biol. 2008 Mar;27(3):151-7. [Abstract]

  • Ling P, Lu TJ, Yuan CJ, Lai MD. (2008) Biosignaling of mammalian Ste20-related kinases. Cell Signal. 2008 Jul;20(7):1237-47. Epub 2008 Jan 4. Review. [Abstract]

  • Hsieh YH, Su IJ, Wang HC, Tsai JH, Huang YJ, Chang WW, Lai MD, Lei HY, Huang W. (2007) Hepatitis B virus pre-S2 mutant surface antigen induces degradation of cyclin-dependent kinase inhibitor p27Kip1 through c-Jun activation domain-binding protein 1. Mol Cancer Res. 2007 Oct;5(10):1063-72. Erratum in: Mol Cancer Res. 2007 Nov;5(11):1223. [Abstract]

  • Hsieh YH, Su IJ, Lei HY, Lai MD, Chang WW, Huang W. (2007) Differential endoplasmic reticulum stress signaling pathways mediated by iNOS. Biochem Biophys Res Commun. 2007 Aug 3;359(3):643-8. Epub 2007 May 30. [Abstract]

  • Lin CC, Tu CF, Yen MC, Chen MC, Hsieh WJ, Chang WC, Chang WT, Lai MD. (2007) Inhibitor of Heat-shock Protein 90 Enhances the Antitumor Effect of DNA Vaccine Targeting Clients of Heat-shock Protein. Mol Ther. 2007 Feb;15(2):404-10. [Abstract]

  • Shiau AL, Lin YP, Shieh GS, Su CH, Wu WL, Tsai YS, Cheng CW, Lai MD, Wu CL. (2007) Development of a Conditionally Replicating Pseudorabies Virus for HER-2/neu-overexpressing Bladder Cancer Therapy. Mol Ther. 2007 Jan;15(1):131-8. [Abstract]

  • Tu CF, Lin CC, Chen MC, Ko TM, Lin CM, Wang YC, Lai MD. (2007) Autologous neu DNA vaccine can be as effective as xenogenic neu DNA vaccine by altering administration route. Vaccine. 2007 Jan 8;25(4):719-28. Epub 2006 Aug 22. [Abstract]

  • Lu TJ, Lai WY, Huang CY, Hsieh WJ, Yu JS, Hsieh YJ, Chang WT, Leu TH, Chang WC, Chuang WJ, Tang MJ, Chen TY, Lu TL, Lai MD. (2006) Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein-tyrosine phosphatase-PEST. J Biol Chem. 2006 Dec 15;281(50):38405-17. Epub 2006 Oct 17. [Abstract]

  • Wang HC, Huang W, Lai MD, Su IJ. (2006) Hepatitis B virus pre-S mutants, endoplasmic reticulum stress and hepatocarcinogenesis. Cancer Sci. 2006 Aug;97(8):683-8. Review. [Abstract]

  • Chang WW, Su IJ, Lai MD, Chang WT, Huang W, Lei HY. (2005) Toll-like receptor 4 plays an anti-HBV role in a murine model of acute hepatitis B virus expression. World J Gastroenterol. 2005 Nov 14;11(42):6631-7. [Abstract]

  • Cheng TL, Chang WW, Su IJ, Lai MD, Huang W, Lei HY, Chang WT. (2005) Therapeutic inhibition of hepatitis B virus surface antigen expression by RNA interference. Biochem Biophys Res Commun. 2005 Oct 28;336(3):820-30. [Abstract]

  • Huang TJ, Lu CC, Tsai JC, Yao WJ, Lu X, Lai MD, Liu HS, Shiau AL. (2005) Novel autoregulatory function of hepatitis B virus M protein on surface gene expression. J Biol Chem. 2005 Jul 29;280(30):27742-54. Epub 2005 May 16. [Abstract]

  • Lu TJ, Huang CY, Yuan CJ, Lee YC, Leu TH, Chang WC, Lu TL, Jeng WY, Lai MD. (2005) Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. J Inorg Biochem. 2005 Jun;99(6):1306-13. [Abstract]

  • Lai MD, Lin WC, Sun YM, Chang FL. (2005) Phosphorylated and hypoacetylated mutant p53 enhances cisplatin-induced apoptosis through caspase-9 pathway in the absence of transcriptional activation or translation. Int J Mol Med. 2005 Apr;15(4):725-34. [Abstract]

  • Wang HC, Chang WT, Chang WW, Wu HC, Huang W, Lei HY, Lai MD, Fausto N, Su IJ. (2005) Hepatitis B virus pre-S2 mutant upregulates cyclin A expression and induces nodular proliferation of hepatocytes. Hepatology. 2005 Feb 22;41(4):761-770 [Epub ahead of print] [Abstract]

  • Hung JH, Su IJ, Lei HY, Wang HC, Lin WC, Chang WT, Huang W, Chang WC, Chang YS, Chen CC, Lai MD. (2004) Endoplasmic reticulum stress stimulates the expression of cyclooxygenase-2 through activation of NF-kappaB and pp38 mitogen-activated protein kinase. J Biol Chem. 2004 Nov 5;279(45):46384-92. Epub 2004 Aug 19. [Abstract]

  • Lin CC, Chou CW, Shiau AL, Tu CF, Ko TM, Chen YL, Yang BC, Tao MH, Lai MD. (2004) Therapeutic HER2/Neu DNA vaccine inhibits mouse tumor naturally overexpressing endogenous neu. Mol Ther. 2004 Aug;10(2):290-301. [Abstract]

  • Hsieh YH, Su IJ, Wang HC, Chang WW, Lei HY, Lai MD, Chang WT, Huang W. (2004) Pre-S mutant surface antigens in chronic hepatitis B virus infection induce oxidative stress and DNA damage. Carcinogenesis. 2004 Oct;25(10):2023-32. Epub 2004 Jun 03. [Abstract]

  • Tsai WC, Tsai ST, Ko JY, Jin YT, Li C, Huang W, Young KC, Lai MD, Liu HS, Wu LW. (2004) The mRNA profile of genes in betel quid chewing oral cancer patients. Oral Oncol. 2004 Apr;40(4):418-26. [Abstract]

  • Chang WW, Su IJ, Lai MD, Chang WT, Huang W, Lei HY. (2003) The role of inducible nitric oxide synthase in a murine acute hepatitis B virus (HBV) infection model induced by hydrodynamics-based in vivo transfection of HBV-DNA. J Hepatol. 2003 Nov;39(5):834-42. [Abstract]

  • Hsieh, J.L., Wu, C.L., Lai, M.D., Lee, C.H., and Shiau, A.L. (2003) Gene therapy for bladder cancer using E1B-55 kD-deleted adenovirus in combination with adenoviral vector encoding plasminogen kringles 1-5. Br J Cancer. 2003 May 6;88(9):1492-9. [Abstract]

  • Guo YL, Chang HC, Tsai JH, Huang JC, Li C, Young KC, Wu LW, Lai MD., Liu HS, Huang W. (2002) Two UVC-induced stress response pathways in HeLa cells identified by cDNA microarray. Environ Mol Mutagen. 2002;40(2):122-8. [Abstract]

  • Huang CY, Wu YM, Hsu CY, Lee WS, Lai MD., Lu TJ, Huang CL, Leu TH, Shih HM, Fang HI, Robinson DR, Kung HJ, Yuan CJ. (2002) Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis. J Biol Chem. 2002 Sep 13;277(37):34367-74. [Abstract]

  • Chang FL, Lai MD. (2001) Various forms of mutant p53 confer sensitivity to cisplatin and doxorubicin in bladder cancer cells. J Urol. 2001 Jul;166(1):304-10. [Abstract]

  • Su, S.-J., Lai, M.-D., Yeh, T.-M., Chow, N.-H. (2001) Overexpression of HER-2/neu enhances the sensitivity of human bladder cancer cells to urinary isoflavones. European Journal of Cancer Volume 37, Issue 11, 2001, Pages 1413-1418 [Abstract]

  • Chen CC, Shieh B, Jin YT, Liau YE, Huang CH, Liou JT, Wu LW, Huang W, Young KC, Lai MD, Liu HS, Li C. (2001) Microarray profiling of gene expression patterns in bladder tumor cells treated with genistein. J Biomed Sci. 2001 Mar-Apr;8(2):214-22. [Abstract]

  • Chen SA, Tsai MH, Wu FT, Hsiang A, Chen YL, Lei HY, Tzai TS, Leung HW, Jin YT, Hsieh CL, Hwang LH, Lai MD. (2000) Induction of antitumor immunity with combination of HER2/neu DNA vaccine and interleukin 2 gene-modified tumor vaccine. Clin Cancer Res. 2000 Nov;6(11):4381-8. [Abstract]

  • Chang FL, Lai MD. (2000) The relationship between p53 status and anticancer drugs-induced apoptosis in nine human bladder cancer cell lines. Anticancer Res. 20(1A):351-5. [Abstract]

  • Chang FL, Ling YF, Lai MD. (2000) Exogenous mutant p53 DNA enhanced cisplatin-induced apoptosis in TSGH-8301 human bladder cancer cells. Anticancer Res. 20(1A):329-36. [Abstract]

  • Hong, M., Lai, MD., Lin, YS. and Lai MZ. (1999) Antagonism of p53-dependent apoptosis by mitogen signals. Cancer Research. 59(12):2847-52. [Abstract]

  • Wang SL. Lai MD. Huang JD. (1999) G169R mutation diminishes the metabolic activity of CYP2D6 in Chinese. Drug Metabolism & Disposition. 27(3):385-8. [Abstract]

  • Huang JD. Guo WC. Lai MD. Guo YL. Lambert GH. (1999) Detection of a novel cytochrome P-450 1A2 polymorphism (F21L) in Chinese. Drug Metabolism & Disposition. 27(1):98-101. [Abstract]

  • Su HJ. Guo YL. Lai MD. Huang JD. Cheng Y. Christiani DC. (1998) The NAT2* slow acetylator genotype is associated with bladder cancer in Taiwanese, but not in the Black Foot Disease endemic area population. Pharmacogenetics. 8(2):187-90

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    Updated: 29 May 2012