Dr. Chang-Shi Chen
Tel:(886)-6-2353535 Ext: 5548 (Office), 5678 (Lab.)
This laboratory studies the genetic bases of the intrinsic cell defense systems against pathogenic bacteria and their virulence factors. We apply chemical genomic, forward/reverse genetic, molecular and biochemical methodologies, and the model organism Caenorhabditis elegans to elucidate the conserved signaling pathways in host-pathogen interactions. The advantage of using C. elegans as a host-pathogen model includes: 1) this organism has a short 2-3 weeks life span; 2) it has a relative small and fully sequenced genome which can facilitates genetic and genomic analysis; 3) a wealth of data has demonstrated that a variety of developmental, neurological, cell biological and biochemical processes have been highly conserved between this simple model organism and mammals. Moreover, a rapidly growing number of human and animal microbial pathogens have been shown to intoxicate and kill C. elegans. In many cases, microbial genes known to be essential for full virulence in mammalian models have
been shown to be similarly required for maximum pathogenicity. Since the innate immune responses are highly conserved among different organisms throughout evolution, understanding the molecular basis of the intrinsic cell defense systems in C. elegans should shed light onto some aspects of immunity in Human diseases.
C. elegans core Taiwan
Han-Sheng Chuang, Hsiang-Yu Chen, Chang-Shi Chen, Wen-Tai Chiu. (2013) Immobilization of the Nematode Caenorhabditis elegans with Addressable Light-Induced Heat Knockdown (ALINK). Lab Chip. 2013 Apr. (accepted)
Ya-Luen Yang, Po-Hsien Huang, Hao-Chieh Chiu, Samuel K. Kulp, Ching-Shih Chen, Cheng-Ju Kuo, Huan-Da Chen, and Chang-Shi Chen. (2013) Histone deacetylase inhibitor AR42 regulates telomerase activity in human glioma cells via an Akt-dependent mechanism. Biochem Biophys Res Commun. 2013 Apr. doi:10.1016/j.bbrc.2013.04.049 (in press)
Chou TC, Chiu HC, Kuo CJ, Wu CM, Syu WJ, Chiu WT, Chen CS. (2013) Enterohaemorrhagic Escherichia coli O157:H7 Shiga-like toxin 1 is required for full pathogenicity and activation of the p38 mitogen-activated protein kinase pathway in Caenorhabditis elegans. Cell Microbiol. 2013 Jan;15(1):82-97. doi: 10.1111/cmi.12030. Epub 2012 Oct 9.
PMID: 22985085 [Abstract]
Ya-Luen Yang, Kah-Sin Loh, Bang-Yu Liou, I-Hua Chu, Cheng-Ju Kuo, Huan-Da Chen, and Chang-Shi Chen. (2012) SESN-1 is a positive regulator of lifespan in Caenorhabditis elegans. Exp Gerontol. 2013 Jan. 11; DOI:10.1016/j.exger.2012.12.011 [Abstract] or original
Wu CJ, Wang HC, Chen CS, Shu HY, Kao AW, Chen PL, Ko WC. (2012) Genome Sequence of a Novel Human Pathogen, Aeromonas aquariorum. J Bacteriol. 2012 Aug;194(15):4114-5.
Chen CS, Bellier A, Kao CY, Yang YL, Chen HD, Los FC, Aroian RV. (2010) WWP-1 is a novel modulator of the DAF-2 insulin-like signaling network involved in pore-forming toxin cellular defenses in Caenorhabditis elegans. PLoS One. 2010 Mar 2;5(3):e9494. [Abstract]
Bellier A, Chen CS, Kao CY, Cinar HN, Aroian RV. (2009) Hypoxia and the Hypoxic Response Pathway Protect against Pore-Forming Toxins in C. elegans. PLoS Pathog. 2009 Dec;5(12):e1000689. Epub. [Abstract]
Xiang-Qian Li, Anderson Tan, Michael Voegtline, Senait Bekele, Chang-Shi Chen and Raffi V. Aroian (2008) Expression of Cry5B protein from Bacillus thuringiensis in plant roots confers resistance to root-knot nematode. Biological Control. Volume 47, Issue 1, October 2008, Pages 97-102
Chen CS, Wang YC, Yang HC, Huang PH, Kulp SK, Yang CC, Lu YS, Matsuyama S, Chen CY, Chen CS. (2007) Histone deacetylase inhibitors sensitize prostate cancer cells to agents that produce DNA double-strand breaks by targeting Ku70 acetylation. Cancer Res. 2007 Jun 1;67(11):5318-27. [Abstract]
Yang CC, Wang YC, Wei S, Lin LF, Chen CS, Lee CC, Lin CC, Chen CS. (2007) Peroxisome proliferator-activated receptor gamma-independent suppression of androgen receptor expression by troglitazone mechanism and pharmacologic exploitation. Cancer Res. 2007 Apr 1;67(7):3229-38. [Abstract]
Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS. (2006) Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor. Mol Pharmacol. 2006 Nov;70(5):1534-41. Epub 2006 Aug 3. [Abstract]
Kulp SK, Chen CS, Wang DS, Chen CY, Chen CS. (2006) Antitumor effects of a novel phenylbutyrate-based histone deacetylase inhibitor, (S)-HDAC-42, in prostate cancer. Clin Cancer Res. 2006 Sep 1;12(17):5199-206. [Abstract]
Yang CC, Ku CY, Wei S, Shiau CW, Chen CS, Pinzone JJ, Ringel MD, Chen CS. (2006) Peroxisome proliferator-activated receptor gamma-independent repression of prostate-specific antigen expression by thiazolidinediones in prostate cancer cells. Mol Pharmacol. 2006 May;69(5):1564-70. Epub 2006 Feb 1. [Abstract]
Lin HY*, Chen CS*, Lin SP, Weng JR, Chen CS. (2006) Targeting histone deacetylase in cancer therapy. Med Res Rev. 2006 Jul;26(4):397-413. Review. (* equal contributions to this article) [Abstract]
Chen CS, Weng SC, Tseng PH, Lin HP, Chen CS. (2005) Histone acetylation-independent effect of histone deacetylase inhibitors on Akt through the reshuffling of protein phosphatase 1 complexes. J Biol Chem. 2005 Nov 18;280(46):38879-87. Epub 2005 Sep 26. [Abstract]
Lu Q, Wang DS, Chen CS, Hu YD, Chen CS. (2005) Structure-based optimization of phenylbutyrate-derived histone deacetylase inhibitors. J Med Chem. 2005 Aug 25;48(17):5530-5. [Abstract]
Shiau CW, Yang CC, Kulp SK, Chen KF, Chen CS, Huang JW, Chen CS. (2005) Thiazolidenediones mediate apoptosis in prostate cancer cells in part through inhibition of Bcl-xL/Bcl-2 functions independently of PPARgamma. Cancer Res. 2005 Feb 15;65(4):1561-9. [Abstract]
Lin HP, Kulp SK, Tseng PH, Yang YT, Yang CC, Chen CS, Chen CS. (2004) Growth inhibitory effects of celecoxib in human umbilical vein endothelial cells are mediated through G1 arrest via multiple signaling mechanisms. (2004) Mol Cancer Ther. 2004 Dec;3(12):1671-80. [Abstract]
Roychowdhury S, Baiocchi RA, Vourganti S, Bhatt D, Blaser BW, Freud AG, Chou J, Chen CS, Xiao JJ, Parthun M, Chan KK, Eisenbeis CF, Ferketich AK, Grever MR, Chen CS, Caligiuri MA. (2004) Selective efficacy of depsipeptide in a xenograft model of Epstein-Barr virus-positive lymphoproliferative disorder. J Natl Cancer Inst. 2004 Oct 6;96(19):1447-57. [Abstract]
Lu Q, Yang YT, Chen CS, Davis M, Byrd JC, Etherton MR, Umar A, Chen CS. (2004) Zn2+-chelating motif-tethered short-chain fatty acids as a novel class of histone deacetylase inhibitors. J Med Chem. 2004 Jan 15;47(2):467-74. [Abstract]
Yang CC, Lin HP, Chen CS, Yang YT, Tseng PH, Rangnekar VM, Chen CS. (2003) Bcl-xL mediates a survival mechanism independent of the phosphoinositide 3-kinase/Akt pathway in prostate cancer cells. J Biol Chem. 2003 Jul 11;278(28):25872-8. Epub 2003 May 8. [Abstract]
Yu SL, Ko KL, Chen CS, Chang YC, Syu WJ. (2000) Characterization of the distal tail fiber locus and determination of the receptor for phage AR1, which specifically infects Escherichia coli O157:H7. J Bacteriol. 2000 Nov;182(21):5962-8. [Abstract]
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Updated: 14 Jan. 2013