NCKU, Department of Cell Biology and Anatomy

Meei-Jyh Jiang

Education

· Ph.D. (1985). University of Cincinnat

· MS (1978). National Taiwan University, Taiwan

· BS (1976). National Taiwan University, Taiwan

Address:

No.1, University Road, Tainan, TAIWAN

Department of Cell Biology and Anatomy, School of Medicine,

National Cheng-Kung University

 

To contact us:

Office: 886-6-2353535 ext 5331

Lab: 886-6-2353535 ext 5309

Fax: 886-6-2093007

E-mail: mjiang@mail.ncku.edu.tw

 

Research Interests

A major focus of my research has been on the signaling pathways for the excitation-contraction coupling of vascular smooth muscle. Regulation of smooth muscle contraction involves multiple pathways and extensive fine tuning and underlies vascular disorders including hypertension and atherosclerosis. My current research is focused on the roles of a small GTP-binding protein Rho in smooth muscle contractility and on defining its upstream and downstream links in the signaling pathways. Another research aspect of mine has involved the factors contributing to atherogenesis. Since smooth muscle cells, a key player for atherosclerosis development, undergo significant modification during atherogenesis, a future direction is heading towards smooth muscle development and differentiation.

 

SPECIALTY :

Muscle and vascular physiology; Cellualr physiology.

Professional Experiences

· Associate Professor (1995-Current). NCKU

Publication

1. Huang YL, Wu CM, Shi GY, Wu GC, Lee H, Jiang MJ, Wu HL, Yang HY. (2009). Nestin serves as a prosurvival determinant that is linked to the cytoprotective effect of epidermal growth factor in rat vascular smooth muscle cells. J Biochem, in press.

2. Huang YL, Shi GY, Lee H, Jiang MJ, Huang BM, Wu HL, Yang HY. (2009). Thrombin induces nestin expression via the transactivation of EGFR signalings in rat vascular smooth muscle cells. Cell Signal. 21:954-968.

3. Lo IC, Lin TM, Chou LH, Liu SL, Wu LW, Shi GY, Wu HL, Jiang MJ. (2009). Ets-1 mediates platelet-derived growth factor-BB-induced thrombomodulin expression in human vascular smooth muscle cells. Cardiovasc Res. 81:771-779.

4. Huang YL, Shi GY, Jiang MJ, Lee H, Chou YW, Wu HL, Yang HY. (2008). Epidermal growth factor up-regulates the expression of nestin through the Ras-Raf-ERK signaling axis in rat vascular smooth muscle cells. Biochem Biophys Res Commun. 377:361-366.

5. Hu HT, Huang YH, Chang YA, Lee CK, Jiang MJ, Wu LW. (2008). Tie2-R849W mutant in venous malformations chronically activates a functional STAT1 to modulate gene expression. J Invest Dermatol. 128:2325-2333.

6. Lam CF, Liu YC, Tseng FL, Sung YH, Huang CC, Jiang MJ, Tsai YC. (2007). High-dose morphine impairs vascular endothelial function by increased production of superoxide anions. Anesthesiology. 106:532-537.

7. Liu SL, Li YH, Shi GY, Jiang MJ, Chang JH, Wu HL. (2007). The effect of statin on the aortic gene expression profiling. Int J Cardiol. 114(1):71-77.

8. Tsai MH, Jiang MJ. (2006). Rho-kinase-mediated regulation of receptor-agonist-stimulated smooth muscle contraction. Pflugers Arch. 453:223-232.

9. Chen WY, Cheng BC, Jiang MJ, Hsieh MY, Chang MS. (2006). IL-20 is expressed in atherosclerosis plaques and promotes atherosclerosis in apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 26:2090-2095.

10. Liu SL, Li YH, Shi GY, Jiang MJ, Chang JH, Wu HL. (2006). The effect of statin on the aortic gene expression profiling. Int J Cardiol. (in press).

11. Hsieh MY, Chen WY, Jiang MJ, Cheng BC, Huang TY, Chang MS. (2006). Interleukin-20 promotes angiogenesis in a direct and indirect manner. Genes Immun. 7:234-242.

12. Liou YM, Chang LY, Liaw JM, Jiang MJ. (2005). Osteopontin gene expression in the aorta and the heart of propylthiouracil-induced hypothyroid mice. J Biomed Sci. 12:869-880.

13. Lo IC, Shih JM, Jiang MJ. (2005). Reactive oxygen species and ERK 1/2 mediate monocyte chemotactic protein-1-stimulated smooth muscle cell migration. J Biomed Sci. 12: 377-388.

14. Tsai MH, Jiang MJ. (2005). Extracellular signal-regulated kinase1/2 in contraction of vascular smooth muscle. Life Sci. 76: 877-888.

15. Liou YM, Jiang MJ, Wu MC (2002). Changes in thiol reactivity and extractability of myofibril bound cardiac troponin C in porcine malignant hyperthermia. J Biochem (Tokyo). 132: 317-325.

16. Lai MD, Jiang MJ, Wing LY (2002). Estrogen stimulates expression of p21Waf1/Cip1 in mouse uterine luminal epithelium. Endocrine. 17:233-239.

17. Yang AL, Tsai SJ, Jiang MJ, Jen CJ, Chen HI (2002).Chronic exercise increases both inducible and endothelial nitric oxide synthase gene expression in endothelial cells of rat aorta. J Biomed Sci. 9: 149-155.

18. Liou YM, Jiang MJ, Wu MC (2000). Altered expression of cardiac myosin isozymes associated with the malignant hyperthermia genotype in swine. Anesthesiology. 93: 1312-1319.

19. Chen YL, Wu HW, Jiang MJ. (2000). Transforming growth factor-beta1 gene and protein expression associated with atherogenesis of cholesterol-fed rabbits. Histol Histopathol. 15: 421-428.

20. Lin TM, Jiang MJ, Eng HL, Shi GY, Lai LC, Huang BJ, Huang KY, Wu HL (2000). Experimental infection with bovine herpesvirus-4 enhances atherosclerotic process in rabbits. Lab Invest. 80: 3-11.

21. Jiang MJ, Yu YJ, Chen YL, Lee YM, Hung LS (1999). Cyclic strain stimulates monocyte chemotactic protein-1 mRNA expression in smooth muscle cells. J Cell Biochem. 76: 303-310.

22. Chen YL, Chang YJ, Jiang MJ. (1999). Monocyte chemotactic protein-1 gene and protein expression in atherogenesis of hypercholesterolemic rabbits. Atherosclerosis. 143: 115-123.

23. Chang YL, Usami S, Hsieh MT, Jiang MJ. (1999). Effects of palmatine on isometric force and [Ca2+]i of arterial smooth muscle. Life Sci. 64: 597-606.

24. Chen HI, Wu YI, Hsieh YL, Shi GY, Jiang MJ, Chang WC, Chuang WJ, Kan WM, Tang MJ, Jen CJ (1997). Perturbation of platelet adhesion to endothelial cells by plasminogen activation in vitro. Thromb Haemost. 78: 934-938.

25. Wang SM, Tsai YJ, Jiang MJ, Tseng YZ (1997). Studies on the function of rho A protein in cardiac myofibrillogenesis. J Cell Biochem. 66: 43-53.

26. Pang JHS, Jiang MJ, Chen YL, Wang FW, Wang DL, Chau LY (1996). Increased ferritin gene expression in atherosclerotic lesions. J Clin Invest. 97: 2204-2212.

27. King L, Jiang MJ, Huang TS, Sheu GC (1995). The protease-susceptible sites and the properties of fragments of aorta smooth muscle myosin. Biochem J. 312: 511-518.

28. Cheng HF, Jiang MJ, Chen CL, Liu SM, Wong LP, Lomasney JW, King K (1995). Cloning and identification of amino acid residues of human phospholipase C1 essential for catalysis. J Biol Chem. 270: 5495-5505.

29. Jiang MJ, King L, Chao YJ (1994). Conformationally altered aortic myosin light chain. Mol Cell Biochem. 136: 113-116.

30. Jiang MJ, Chan CF, Chang YL (1994). Changes in [Ca2+]i and myosin light chain phosphorylation during U46619-activated contraction. Life Sci. 54: 2005-2013.

31. Wing LYC, Chan CF, Jiang MJ. (1993). Acetylpolyamines decrease blood pressure, [Ca2+]i and isometric force of vascular smooth muscle. J Pharmacol Exp Ther. 266: 179-183.

32. Jiang MJ, Morgan KG (1989). Agonist-specific myosin phosphorylation and intracellular calcium during isometric contractions of arterial smooth muscle. Pflugers Archiv. 413: 637-643.

33. Jiang MJ, Morgan KG (1987). Intracellular calcium levels in phorbol ester-induced contractions of vascular muscle. Am J Physiol. 253 (Heart Circ Physiol 22): H1365-H1371.

　

 

CONFERENCE PAPERS (2001-2005) :

 

1. Chen YT, Jiang MJ, Wing LYC, Lee YH, Cheng KS, Liu CC (2005). Microscopic image analysis for characterizing the atherosclerosis in apoE knockout mouse. Proc Int Fed Med Biol Engineer 8: PA-3-04.

2. Chen YT, Liu CC, Jiang MJ, Wing LYC, Lee YH, Cheng KS (2003). Aorta image analysis and characterization for atherosclerosis of apoE knockout (ko) mice. Biomedical engineering society annual symposium, PIII-49.

 

SYMPOSIUM ABSTRACTS (2001-2006):

 

1. Shih YY, Jiang MJ, Wing LYC (2005). Effects of estrogen and progestin on atherosclerotic progression and lipid profile in apoE-/- mice. The Twentieth Joint Annual Conference of Biomedical Sciences, O48.

2. Chen YC, Shih YY, Wing LYC, Jiang MJ (2005). Estrogen treatment inhibits atherosclerosis progression and superoxide production in female apoE-deficient mice. The Twentieth Joint Annual Conference of Biomedical Sciences, P362.

3. Cheng JC, Jiang MJ (2005). The phosphorylation and expression of aortic myosin phosphatase during atherosclerosis progression. The Twentieth Joint Annual Conference of Biomedical Sciences, P361.

4. Lee CL, Shiao MS, Jiang MJ (2005). Effects of Salvinolic acid B on growth factors-induced migration of rat aortic smooth muscle cells. The Twentieth Joint Annual Conference of Biomedical Sciences, P360.

5. Chen YH, So EC, Chou NS, Cheng BC, Wong KL, Jiang MJ (2004). The Characterization of Serum Requirement for Primary Culture of Human Aortic Smooth Muscle Cell. Acta Anaesthesiol Taiwanica 42S: L36.

6. Cheng JC, Jiang MJ (2004). The expression and phosphorylation of aortic myosin phosphatase during atherosclerosis progression. The Nineteenth Joint Annual Conference of Biomedical Sciences, P301.

7. Hung CL, Jiang MJ (2003). The roles of rhoA and extracellular matrix proteins on the expression of smooth muscle differentiation markers. International symposium on vascular physiology, Tainan, Taiwan, P17.

8. Lee YH, Chen YT, Liu CC, Jiang MJ (2003). The effects of rho kinase inhibition on atherosclerosis progression and smooth muscle contractility of apoE-deficient mice. International symposium on vascular physiology, Tainan, Taiwan, P7.

9. Hung CL, Jiang MJ (2003). Studies on smooth muscle cell phenotype modulation by extracellular matrix proteins. The Eighteenth Joint Annual Conference of Biomedical Sciences, P205.

10. Shih JM, Lo IC, Jiang MJ (2003). The signaling pathways of MCP-1-induced smooth muscle cell migration. The Eighteenth Joint Annual Conference of Biomedical Sciences, P204.

11. Lo HG, Lee CL, Tsai MH, Shih JM, Jiang MJ (2002). The roles of RhoA in actin cytoskeleton organization of vascular smooth muscle cells. FASEB Summer Research Conference on “Small G-Proteins and Cell Dynamics”, July 6-11, 2002.

12. Shih JM, Lo IC, Jiang MJ (2002). The signaling pathways of MCP-1-induced smooth muscle cell migration. The Seventeenth Joint Annual Conference of Biomedical Sciences, P186.

13. Tsai MH, Jiang MJ (2002). Roles of tyrosine kinases and extracellular signal-regulated kinases in receptor-coupled Ca2+-sensitization of arterial smooth muscle. The Seventeenth Joint Annual Conference of Biomedical Sciences, P185.

14. Tsai MH, Jiang MJ (2002). Rho kinase, tyrosine kinases and extracellular signal-regulated kinases in Ca2+-sensitization of vascular smooth muscle. FASEB J 16: A387.

15. Jiang MJ, Lo IC, Shih JM (2002). The effects of monocyte chemotactic protein-1 on smooth muscle cell migration. Biophys J 82: 424a.

 

RESEARCH GRANTS AWARDED (past five years):

 

1. 2005-2006 “Inflammation-induced responses of smooth muscle cells in vascular remodeling – the roles of thrombomodulin family members”, sub-project investigator of “Program project on vascular biology”, supported by Program for Promoting University Academic Excellence, Ministry of Education, R.O.C..

Students


Master Degree

            蔡怡青 (成大細胞生物與解剖所, 2009--)

            白濬芃 (成大細胞生物與解剖所, 2008--)

黃彥文 (成大細胞生物與解剖所, 2007-2009)

吳彥廷 (成大細胞生物與解剖所, 2007-2010)

潘子茜 (成大細胞生物與解剖所, 2007-2009)

李秋慧 (成大細胞生物與解剖所, 2006-2009)

            林伯庭 (成大細胞生物與解剖所, 2006-2008) c-Jun N-terminal kinase 在血管收縮素二所

                           誘發血管皮滑肌細胞老化的角色           

            林伯祐 (成大細胞生物與解剖所, 2006-2008) 第一型膠原蛋白的聚合狀態對血管平滑肌

                         細胞表現型的影響

周凌慧 (成大細胞生物與解剖所, 2004-2007) 小鼠頸動脈結紮模式中血管平滑肌細胞型態轉變過程之研究

鄔孟陵 (成大細胞生物與解剖所, 2005-2007) 細胞外基質合成酵素lysyl oxidase和prolyl 4-hydroxylase在人類腹主動脈瘤平滑肌細胞的表現

鄭融鍵 (成大細胞生物與解剖所, 2005年畢) 在apoE基因剔除小鼠動脈粥狀硬化發展過程中主動脈之肌凝蛋白去磷酸?表現量的變化

李佳玲 (成大細胞生物與解剖所, 2005年畢) 探討丹參酚酸B對血管平滑肌細胞的移行與增生能力之影響

陳應輝 (成大細胞生物與解剖所, 2005年畢) 人類腹主動脈瘤血管平滑肌細胞之反應性氧化產物的生成及NAD(P)H氧化?的表現

陳亞琪 (成大細胞生物與解剖所, 2005年畢) 雌激素處理對apoE基因剔除母鼠活性氧分子的產生與動脈粥狀硬化進程之影響

李宜倖 (成大細胞生物與解剖所, 2004年畢) Rho蛋白激酶的抑制對apoE基因剔除小鼠動脈粥狀硬化的病程發展與收縮力的影響

洪萩菱 (成大生理所, 2004年畢) RhoA和細胞外間質蛋白在平滑肌細胞分化指標蛋白表現上所扮演的角色

施俊名 (成大細胞生物與解剖所, 2003年畢) 單核球趨化蛋白-1刺激血管平滑肌細胞移行作用機制之探討

羅鴻碁 (成大生理所, 2002年畢) TAT-HA-RhoA融合突變蛋白的表現;純化;及其在血管平滑肌細胞之胞骨骼重組的角色

羅翊中 (成大生理所, 2001年畢) 單核球趨化蛋白-1在血管管平滑肌細胞移行的作用

 

PhD Degree

     吳彥廷 (成大基醫所博士班, 2010—-)

羅翊中 (成大基醫所博士班, 2003-2009)

蔡明和 (成大基醫所, 2006年畢) ERK1/2, Rho-kinase 及NADPH oxidase 衍生之活性氧物質在血管平滑肌興奮-收縮聯結之調控角色

Hobbies

· Reading, hiking, music listening, chatting